Methods in Molecular Biology

Neutrophil Methods and Protocols

Editors: Quinn, Mark T., DeLeo, Frank R. (Eds.)

  • Provides neutrophil protocols that are broadly applicable to leukocyte biology
  • Includes protocols for isolation of neutrophils from humans and animal species
  • Presents an interesting and informative overview of the field of neutrophil biology
  • Facilitates a better understanding of the role of neutrophils in host defense
see more benefits

Buy this book

eBook $119.00
price for USA (gross)
  • ISBN 978-1-62703-845-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.00
price for USA
  • ISBN 978-1-62703-844-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA
  • ISBN 978-1-4939-6062-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. Neutrophil Methods and Protocols, Second Edition provides a concise set of protocols written by leading researchers in the field for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step diagnostic assays of common neutrophil disorders. Topics covered include an overview of neutrophils and their role in host defense and inflammation; methods most commonly used for isolating neutrophils from humans and other animal species; procedures for subcellular fractionation of human neutrophils, analysis of in vivo transmigrated neutrophils, generation of mature neutrophils from induced pluripotent stem cells and analysis of neutrophil gene expression; methods for investigating priming, oxidant production, phagocytosis, bactericidal activity and extracellular trap formation and protocols for investigating neutrophil adhesion, chemotaxis and outside-in signaling via integrins. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Neutrophil Methods and Protocols, Second Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.

Table of contents (32 chapters)

  • The Role of Neutrophils in the Immune System: An Overview

    Malech, Harry L. (et al.)

    Pages 3-10

  • Isolation of Human Neutrophils from Venous Blood

    Nauseef, William M.

    Pages 13-18

  • Neutrophil Isolation from Nonhuman Species

    Siemsen, Daniel W. (et al.)

    Pages 19-37

  • Collection of In Vivo Transmigrated Neutrophils from Human Skin

    Christenson, Karin (et al.)

    Pages 39-52

  • Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers

    Clemmensen, Stine Novrup (et al.)

    Pages 53-76

Buy this book

eBook $119.00
price for USA (gross)
  • ISBN 978-1-62703-845-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.00
price for USA
  • ISBN 978-1-62703-844-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA
  • ISBN 978-1-4939-6062-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Neutrophil Methods and Protocols
Editors
  • Mark T. Quinn
  • Frank R. DeLeo
Series Title
Methods in Molecular Biology
Series Volume
1124
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-845-4
DOI
10.1007/978-1-62703-845-4
Hardcover ISBN
978-1-62703-844-7
Softcover ISBN
978-1-4939-6062-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 551
Number of Illustrations and Tables
65 b/w illustrations, 28 illustrations in colour
Topics