Methods in Molecular Biology

Metalloproteins

Methods and Protocols

Editors: Fontecilla-Camps, Juan C., Nicolet, Yvain (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
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  • ISBN 978-1-62703-794-5
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Hardcover $119.00
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  • ISBN 978-1-62703-793-8
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  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
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  • ISBN 978-1-4939-6328-7
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About this book

Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.

Table of contents (15 chapters)

  • Advances in Research on Metalloproteins

    Frey, Perry A.

    Pages 1-3

  • Purification of O2-Sensitive Metalloproteins

    Echavarri-Erasun, Carlos (et al.)

    Pages 5-18

  • Expression and Purification of NifB Proteins from Aerobic and Anaerobic Sources

    Echavarri-Erasun, Carlos (et al.)

    Pages 19-31

  • Techniques for the Production, Isolation, and Analysis of Iron–Sulfur Proteins

    Crack, Jason C. (et al.)

    Pages 33-48

  • Cell-Free Synthesis of the H-Cluster: A Model for the In Vitro Assembly of Metalloprotein Metal Centers

    Kuchenreuther, Jon M. (et al.)

    Pages 49-72

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-794-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-793-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6328-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Metalloproteins
Book Subtitle
Methods and Protocols
Editors
  • Juan C. Fontecilla-Camps
  • Yvain Nicolet
Series Title
Methods in Molecular Biology
Series Volume
1122
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-794-5
DOI
10.1007/978-1-62703-794-5
Hardcover ISBN
978-1-62703-793-8
Softcover ISBN
978-1-4939-6328-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 299
Number of Illustrations and Tables
32 b/w illustrations, 57 illustrations in colour
Topics