Methods in Molecular Biology

Ras Signaling

Methods and Protocols

Editors: Trabalzini, Lorenza, Retta, Saverio Francesco (Eds.)

  • Includes experimental approaches to the complexity of the biological functions of RasGTPases
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-791-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-790-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6054-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, Ras Signaling: Methods and Protocols contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioral approaches, advanced high resolution fluorescence and electron microscopy imaging and “omics” technologies. Through this, the detailed volume provides information on expression, post-translational modifications, subcellular localization and dynamics, regulatory mechanisms of upstream and downstream signaling pathways and ultimately, biological activities and functions of Ras GTPases in different model systems, including high and low eukaryotic organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and authoritative, Ras Signaling: Methods and Protocols serves as an aid for investigators of different backgrounds and interests related to the multiple physiological and pathological functions of the large superfamily of Ras GTPases.

Table of contents (26 chapters)

  • The Ras Superfamily of Small GTPases: The Unlocked Secrets

    Goitre, Luca (et al.)

    Pages 1-18

  • A Novel Method for the Production of Fully Modified K-Ras 4B

    Chavan, Tanmay S. (et al.)

    Pages 19-32

  • Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation

    Tsai, Frederick D. (et al.)

    Pages 33-41

  • Ubiquitination of Rac1 by Inhibitors of Apoptosis (IAPs)

    Oberoi-Khanuja, Tripat Kaur (et al.)

    Pages 43-54

  • Ras GTPases Are Both Regulators and Effectors of Redox Agents

    Ferro, Elisa (et al.)

    Pages 55-74

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-791-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-790-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6054-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Ras Signaling
Book Subtitle
Methods and Protocols
Editors
  • Lorenza Trabalzini
  • Saverio Francesco Retta
Series Title
Methods in Molecular Biology
Series Volume
1120
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-791-4
DOI
10.1007/978-1-62703-791-4
Hardcover ISBN
978-1-62703-790-7
Softcover ISBN
978-1-4939-6054-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 425
Number of Illustrations and Tables
31 b/w illustrations, 39 illustrations in colour
Topics