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Methods in Molecular Biology

Human Cytomegaloviruses

Methods and Protocols

Editors: Yurochko, Andrew D., Miller, William E. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-788-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
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  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-787-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6273-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis. 

Table of contents (18 chapters)

  • History of the Molecular Biology of Cytomegaloviruses

    Stinski, Mark F.

    Pages 1-14

  • Overview of Human Cytomegalovirus Pathogenesis

    Nogalski, Maciej T. (et al.)

    Pages 15-28

  • Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies

    Frascaroli, Giada (et al.)

    Pages 29-46

  • Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirus Infection

    Fortunato, Elizabeth A.

    Pages 47-57

  • Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants

    Dekhtiarenko, Iryna (et al.)

    Pages 59-79

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-788-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-787-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6273-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Human Cytomegaloviruses
Book Subtitle
Methods and Protocols
Editors
  • Andrew D. Yurochko
  • William E. Miller
Series Title
Methods in Molecular Biology
Series Volume
1119
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer New York
eBook ISBN
978-1-62703-788-4
DOI
10.1007/978-1-62703-788-4
Hardcover ISBN
978-1-62703-787-7
Softcover ISBN
978-1-4939-6273-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 368
Number of Illustrations and Tables
18 b/w illustrations, 22 illustrations in colour
Topics