Methods in Molecular Biology

Cell-Free Protein Synthesis

Methods and Protocols

Editors: Alexandrov, Kirill, Johnston, Wayne A. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-782-2
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Hardcover $139.00
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Softcover $139.00
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  • ISBN 978-1-4939-6254-9
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About this book

Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies.

Table of contents (22 chapters)

  • Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae

    Johnston, Wayne A. (et al.)

    Pages 1-15

  • Bioinformatics Analysis and Optimization of Cell-Free Protein Synthesis

    Tokmakov, Alexander A. (et al.)

    Pages 17-33

  • A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression

    Kralicek, Andrew

    Pages 35-54

  • One-Pot, Microscale Cell-Free Enzyme Expression and Screening

    Chandrasekaran, Aarthi (et al.)

    Pages 55-69

  • Cell-Free Translation of Biofuel Enzymes

    Takasuka, Taichi E. (et al.)

    Pages 71-95

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-782-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-781-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6254-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cell-Free Protein Synthesis
Book Subtitle
Methods and Protocols
Editors
  • Kirill Alexandrov
  • Wayne A. Johnston
Series Title
Methods in Molecular Biology
Series Volume
1118
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-782-2
DOI
10.1007/978-1-62703-782-2
Hardcover ISBN
978-1-62703-781-5
Softcover ISBN
978-1-4939-6254-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 313
Number of Illustrations and Tables
35 b/w illustrations, 31 illustrations in colour
Topics