Methods in Molecular Biology

DNA Cloning and Assembly Methods

Editors: Valla, Svein, Lale, Rahmi (Eds.)

  • Included methods based on mechanism
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-764-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-763-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6057-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.

With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.

Table of contents (18 chapters)

  • BioBrick Assembly Standards and Techniques and Associated Software Tools

    Røkke, Gunvor (et al.)

    Pages 1-24

  • Plasmid Construction by SLIC or Sequence and Ligation-Independent Cloning

    Hill, Ryan E. (et al.)

    Pages 25-36

  • Quick and Clean Cloning

    Thieme, Frank (et al.)

    Pages 37-48

  • Hierarchical Ligation-Independent Assembly of PCR Fragments

    Schmid-Burgk, Jonathan L. (et al.)

    Pages 49-58

  • USER-Derived Cloning Methods and Their Primer Design

    Salomonsen, Bo (et al.)

    Pages 59-72

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-764-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-763-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6057-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
DNA Cloning and Assembly Methods
Editors
  • Svein Valla
  • Rahmi Lale
Series Title
Methods in Molecular Biology
Series Volume
1116
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, New York
eBook ISBN
978-1-62703-764-8
DOI
10.1007/978-1-62703-764-8
Hardcover ISBN
978-1-62703-763-1
Softcover ISBN
978-1-4939-6057-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 303
Number of Illustrations and Tables
24 b/w illustrations, 50 illustrations in colour
Topics