Methods in Molecular Biology

Gene Correction

Methods and Protocols

Editors: Storici, Francesca (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-761-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-760-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6321-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy.

Table of contents (29 chapters)

  • RecTEPsy-Mediated Recombineering in Pseudomonas syringae

    Swingle, Bryan

    Pages 3-10

  • Genome Manipulations with Bacterial Recombineering and Site-Specific Integration in Drosophila

    Zhang, Yi (et al.)

    Pages 11-24

  • Multiple Genetic Manipulations of DT40 Cell Line

    Motegi, Akira (et al.)

    Pages 25-35

  • Gene Targeting of Human Pluripotent Stem Cells by Homologous Recombination

    Howden, Sara E. (et al.)

    Pages 37-55

  • Methods for the Assessment of ssODN-Mediated Gene Correction Frequencies in Muscle Cells

    Bertoni, Carmen

    Pages 59-83

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-761-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-760-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6321-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Gene Correction
Book Subtitle
Methods and Protocols
Editors
  • Francesca Storici
Series Title
Methods in Molecular Biology
Series Volume
1114
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-761-7
DOI
10.1007/978-1-62703-761-7
Hardcover ISBN
978-1-62703-760-0
Softcover ISBN
978-1-4939-6321-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 463
Number of Illustrations and Tables
28 b/w illustrations, 59 illustrations in colour
Topics