Methods in Molecular Biology

Artificial Riboswitches

Methods and Protocols

Editors: Ogawa, Atsushi (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-62703-755-6
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Hardcover $139.00
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About this book

Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protocols focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, protocols for evaluating the activities of the resultant riboswitches, as well as protocols for construction of ligand-dependent, trans-acting gene regulators. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Artificial Riboswitches: Methods and Protocols seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field.

Table of contents (20 chapters)

  • Computational Design of RNA Libraries for In Vitro Selection of Aptamers

    Chushak, Yaroslav G. (et al.)

    Pages 1-15

  • In Vitro Selection of RNA Aptamers for a Small-Molecule Dye

    Murata, Asako (et al.)

    Pages 17-28

  • Development of Photoswitchable RNA Aptamer–Ligand Complexes

    Hayashi, Gosuke (et al.)

    Pages 29-40

  • Identification of RNA Aptamers Against Recombinant Proteins with a Hexa-Histidine Tag

    Ohuchi, Shoji

    Pages 41-56

  • Artificial Riboswitch Selection: A FACS-Based Approach

    Ghazi, Zohaib (et al.)

    Pages 57-75

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-755-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-754-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6330-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Artificial Riboswitches
Book Subtitle
Methods and Protocols
Editors
  • Atsushi Ogawa
Series Title
Methods in Molecular Biology
Series Volume
1111
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-755-6
DOI
10.1007/978-1-62703-755-6
Hardcover ISBN
978-1-62703-754-9
Softcover ISBN
978-1-4939-6330-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 289
Number of Illustrations and Tables
30 b/w illustrations, 45 illustrations in colour
Topics