Methods in Molecular Biology

Animal Cell Biotechnology

Methods and Protocols

Editors: Pörtner, Ralf (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-733-4
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-62703-732-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
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  • ISBN 978-1-4939-6318-8
  • Free shipping for individuals worldwide
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About this book

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Table of contents (26 chapters)

  • High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidics

    Lee, Wong Cheng (et al.)

    Pages 3-13

  • Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology

    Vidigal, João (et al.)

    Pages 15-27

  • Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine

    Fliedl, Lukas (et al.)

    Pages 29-34

  • Scalable Transient Protein Expression

    Chiou, Henry C. (et al.)

    Pages 35-55

  • DNA Profiling and Characterization of Animal Cell Lines

    Stacey, Glyn N. (et al.)

    Pages 57-73

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-733-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-62703-732-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA
  • ISBN 978-1-4939-6318-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Animal Cell Biotechnology
Book Subtitle
Methods and Protocols
Editors
  • Ralf Pörtner
Series Title
Methods in Molecular Biology
Series Volume
1104
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
Distribution Rights
Distribution rights for India: Scientific International, New Delhi, India
eBook ISBN
978-1-62703-733-4
DOI
10.1007/978-1-62703-733-4
Hardcover ISBN
978-1-62703-732-7
Softcover ISBN
978-1-4939-6318-8
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XIV, 498
Number of Illustrations and Tables
55 b/w illustrations, 28 illustrations in colour
Topics