Methods in Molecular Biology

The Complement System

Methods and Protocols

Editors: Gadjeva, Mihaela (Ed.)

  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-724-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-723-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6306-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols  to purify and analyze the activity of the individual complement components or pathways.  It includes assays that describe detection  of complement SNPs,  clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established “classical” assays and recently developed “new” assays to analyze the complement system activation will be useful to a wide audience of scientists.

Table of contents (32 chapters)

  • Overview

    Gadjeva, Mihaela

    Pages 1-9

  • Evaluation of Complement Function by ELISA

    Roos, Anja (et al.)

    Pages 11-23

  • Classical Complement Pathway Component C1q: Purification of Human C1q, Isolation of C1q Collagen-Like and Globular Head Fragments and Production of Recombinant C1q—Derivatives. Functional Characterization

    Kojouharova, Mihaela

    Pages 25-42

  • Classical Complement Pathway Components C1r and C1s: Purification from Human Serum and in Recombinant Form and Functional Characterization

    Rossi, Véronique (et al.)

    Pages 43-60

  • Expression and Purification Methods for the Production of Recombinant Human Complement Component C2

    Norton, Angela W. (et al.)

    Pages 61-74

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-724-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-723-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6306-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Complement System
Book Subtitle
Methods and Protocols
Editors
  • Mihaela Gadjeva
Series Title
Methods in Molecular Biology
Series Volume
1100
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, New York
eBook ISBN
978-1-62703-724-2
DOI
10.1007/978-1-62703-724-2
Hardcover ISBN
978-1-62703-723-5
Softcover ISBN
978-1-4939-6306-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 383
Number of Illustrations and Tables
54 b/w illustrations, 27 illustrations in colour
Topics