Methods in Molecular Biology

Bioluminescent Imaging

Methods and Protocols

Editors: Badr, Christian E. (Ed.)

  • Includes cutting-edge methods and protocols to examine bioluminescent imaging 
  • Provides step-by-step, easy to follow detail essential for reproduction
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-718-1
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  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-717-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6067-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.

Reviews

From the reviews:

“The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. … The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields.” (Omer Iqbal, Doody's Book Reviews, January, 2014)

Table of contents (20 chapters)

  • Bioluminescence Imaging: Basics and Practical Limitations

    Badr, Christian E.

    Pages 1-18

  • Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells

    Chida, Junji (et al.)

    Pages 21-32

  • Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter

    Tricoire, Ludovic (et al.)

    Pages 33-45

  • Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture

    Degeling, M. Hannah (et al.)

    Pages 47-55

  • Split Gaussia Luciferase for Imaging Ligand–Receptor Binding

    Luker, Kathryn E. (et al.)

    Pages 59-69

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-718-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-717-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6067-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bioluminescent Imaging
Book Subtitle
Methods and Protocols
Editors
  • Christian E. Badr
Series Title
Methods in Molecular Biology
Series Volume
1098
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-718-1
DOI
10.1007/978-1-62703-718-1
Hardcover ISBN
978-1-62703-717-4
Softcover ISBN
978-1-4939-6067-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 276
Number of Illustrations and Tables
11 b/w illustrations, 39 illustrations in colour
Topics