Methods in Molecular Biology

Functional Analysis of DNA and Chromatin

Editors: Stockert, Juan Carlos, Espada, Jesús, Blázquez-Castro, Alfonso (Eds.)

  • Presents an overview of standard and more recent methods for the functional analysis of the genetic material
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-706-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-705-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6048-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister chromatid exchanges, FISH, DNA and chromatin imaging by fluorescence, electron and atomic force microscopy, detection of apoptotic DNA, cytosine methylation and hydroxy-methylation, DNA thermophoresis, improved methods for histone analysis, chromatin immunoprecipitation, and analysis of rDNA genes and chromatin-associated RNA. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Functional Analysis of DNA and Chromatin seeks to aid scientists in the further study of cellular and molecular biology of the nucleus.

Table of contents (28 chapters)

  • Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern

    Stockert, Juan C.

    Pages 1-12

  • Using Microchip Gel Electrophoresis to Probe DNA–Drug Binding Interactions

    Shi, Nan (et al.)

    Pages 13-24

  • Identifying Different Types of Chromatin Using Giemsa Staining

    Stockert, Juan C. (et al.)

    Pages 25-38

  • Analysis of DNA Damage and Repair by Comet Fluorescence In Situ Hybridization (Comet-FISH)

    Glei, Michael (et al.)

    Pages 39-48

  • Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level

    Sestili, Piero (et al.)

    Pages 49-70

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-706-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-705-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6048-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Functional Analysis of DNA and Chromatin
Editors
  • Juan Carlos Stockert
  • Jesús Espada
  • Alfonso Blázquez-Castro
Series Title
Methods in Molecular Biology
Series Volume
1094
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, New York
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-62703-706-8
DOI
10.1007/978-1-62703-706-8
Hardcover ISBN
978-1-62703-705-1
Softcover ISBN
978-1-4939-6048-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 365
Number of Illustrations and Tables
30 b/w illustrations, 44 illustrations in colour
Topics