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miRNA Maturation

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Christoph Arenz

    1. Institute for Chemistry, Humboldt Universität zu Berlin, Berlin, Germany

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  • Seeks to widen the view on miRNA as biological mediator and potential drug target
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1095)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Reviews

    1. Front Matter

      Pages 1-1
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    2. The Pathway of miRNA Maturation

      • Michael Sand
      Pages 3-10

    3. MicroRNA Maturation and Human Disease

      • Marlen Hesse, Christoph Arenz
      Pages 11-25

    4. Approaches to the Modulation of miRNA Maturation

      • Valerie T. Tripp, Jaclyn R. McKenna, Douglas D. Young
      Pages 27-58

  3. Methods

    1. Front Matter

      Pages 59-59
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    2. Expression Profiling of Components of the miRNA Maturation Machinery

      • Michael Sand, Marina Skrygan
      Pages 61-71

    3. Primary MicroRNA Processing Assay Reconstituted Using Recombinant Drosha and DGCR8

      • Ian Barr, Feng Guo
      Pages 73-86

    4. In Vivo Processing Assay Based on a Dual-Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity

      • Vera Bilan, Danilo Allegra, Florian Kuchenbauer, Daniel Mertens
      Pages 87-93

    5. Assaying Dicer-Mediated miRNA Maturation by Means of Fluorescent Substrates

      • Marlen Hesse, Brian P. Davies, Christoph Arenz
      Pages 95-102

    6. A Fluorescence Correlation Spectroscopy-Based Enzyme Assay for Human Dicer

      • Eileen Magbanua, Ulrich Hahn
      Pages 103-108

    7. Detection of microRNA Maturation Using Unmodified pre-microRNA and Branched Rolling Circle Amplification

      • Saskia Neubacher, Christoph Arenz
      Pages 109-119

    8. Quantitative RT-PCR Specific for Precursor and Mature miRNAs

      • Hannah Zöllner, Stephan A. Hahn, Abdelouahid Maghnouj
      Pages 121-134

    9. Cellular MicroRNA Sensors Based on Luciferase Reporters

      • Colleen M. Connelly, Alexander Deiters
      Pages 135-146

    10. Identification of Inhibitors of MicroRNA Function from Small Molecule Screens

      • Colleen M. Connelly, Alexander Deiters
      Pages 147-156

    11. Inhibition of miRNA Maturation by Peptide Nucleic Acids

      • Concetta Avitabile, Enrica Fabbri, Nicoletta Bianchi, Roberto Gambari, Alessandra Romanelli
      Pages 157-164

    12. Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs

      • Eleonora Brognara, Enrica Fabbri, Nicoletta Bianchi, Alessia Finotti, Roberto Corradini, Roberto Gambari
      Pages 165-176

    13. Lentiviral Overexpression of miRNAs

      • Hannah Zöllner, Stephan A. Hahn, Abdelouahid Maghnouj
      Pages 177-190

  4. Back Matter

    Pages 191-192
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Keywords

About this book

In miRNA Maturation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study miRNA maturation. These included established methods such as fluorescent and non-fluorescent methods for homogenous assays of Dicer-mediated miRNA maturation or an in vivo assay for Drosha activity. Moreover, the volume also contains useful, but less-common methods that are hard to find elsewhere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, miRNA Maturation: Methods and Protocols seeks to widen the view on miRNA as biological mediator and potential drug target.

Editors and Affiliations

  • Institute for Chemistry, Humboldt Universität zu Berlin, Berlin, Germany

    Christoph Arenz

Bibliographic Information

  • Book Title: miRNA Maturation

  • Book Subtitle: Methods and Protocols

  • Editors: Christoph Arenz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-703-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-702-0Published: 29 October 2013

  • Softcover ISBN: 978-1-4939-6016-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-703-7Published: 28 October 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 192

  • Number of Illustrations: 21 b/w illustrations, 29 illustrations in colour

  • Topics: Human Genetics, Gene Expression

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