Methods in Molecular Biology

PIWI-Interacting RNAs

Methods and Protocols

Editors: Siomi, Mikiko C. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-694-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-693-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6298-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

PIWI-interacting RNAs (piRNAs) are the third and most-recently discovered group of silencing-inducible small RNAs in animals. PIWI-Interacting RNAs: Methods and Protocols provides the most recent methods and protocols for studying piRNAs in the gonads of a wide range of species, as well as in any other organs where piRNAs may be detected. Comprehensive high-throughput sequencing analysis of piRNAs in embryos, testes and ovaries of D. melanogaster, as well as in mouse and rat testes, has raised the profile of piRNAs and thus further accelerated piRNA studies. In addition to C. elegans, other model species such as Drosophila melanogaster, Arabidopsis thaliana and mice, along with cultured cell lines such as HeLa and Drosophila Schneider 2 (S2), and other such organisms have been used to address the fundamental questions of the biogenesis and functions of RNAi-triggering small non-coding RNAs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, PIWI-Interacting RNAs: Methods and Protocols seeks to serve both established researchers and newcomers to the field to progress towards the ultimate goal of understanding the mechanisms and actions of piRNAs.

Table of contents (20 chapters)

  • Chromatin Immunoprecipitation Assay of Piwi in Drosophila

    Yin, Hang (et al.)

    Pages 1-11

  • Drosophila Germline Stem Cells for In Vitro Analyses of PIWI-Mediated RNAi

    Niki, Yuzo (et al.)

    Pages 13-23

  • RNAi and Overexpression of Genes in Ovarian Somatic Cells

    Saito, Kuniaki

    Pages 25-33

  • Making piRNAs In Vitro

    Kawaoka, Shinpei (et al.)

    Pages 35-46

  • A Framework for piRNA Cluster Manipulation

    Olovnikov, Ivan (et al.)

    Pages 47-58

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-694-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-693-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6298-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
PIWI-Interacting RNAs
Book Subtitle
Methods and Protocols
Editors
  • Mikiko C. Siomi
Series Title
Methods in Molecular Biology
Series Volume
1093
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-694-8
DOI
10.1007/978-1-62703-694-8
Hardcover ISBN
978-1-62703-693-1
Softcover ISBN
978-1-4939-6298-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 249
Number of Illustrations and Tables
25 b/w illustrations, 23 illustrations in colour
Topics