Methods in Molecular Biology

Adenovirus

Methods and Protocols

Editors: Chillón, Miguel, Bosch, Assumpció (Eds.)

  • Includes cutting-edge methods and protocols for adenovirus research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-679-5
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  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-678-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
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  • ISBN 978-1-4939-6325-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics.  Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.

Table of contents (16 chapters)

  • Biophysical Methods to Monitor Structural Aspects of the Adenovirus Infectious Cycle

    Menéndez-Conejero, Rosa (et al.)

    Pages 1-24

  • Proteome Analysis of Adenovirus Using Mass Spectrometry

    Lind, Sara Bergström (et al.)

    Pages 25-44

  • Capsid Modification Strategies for Detargeting Adenoviral Vectors

    Parker, Alan L. (et al.)

    Pages 45-59

  • Use of Dodecahedron “VLPs” as an Alternative to the Whole Adenovirus

    Fender, Pascal

    Pages 61-70

  • Study of Adenovirus and CAR Axonal Transport in Primary Neurons

    Zussy, Charleine (et al.)

    Pages 71-78

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-679-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-678-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6325-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Adenovirus
Book Subtitle
Methods and Protocols
Editors
  • Miguel Chillón
  • Assumpció Bosch
Series Title
Methods in Molecular Biology
Series Volume
1089
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-679-5
DOI
10.1007/978-1-62703-679-5
Hardcover ISBN
978-1-62703-678-8
Softcover ISBN
978-1-4939-6325-6
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XI, 247
Number of Illustrations and Tables
22 b/w illustrations, 15 illustrations in colour
Topics