Methods in Molecular Biology

Human Retroviruses

Methods and Protocols

Editors: Vicenzi, Elisa, Poli, Guido (Eds.)

  • Follows the step-by-step life cycle of a retrovirus
  • Provides state-of-the-art methodological protocols from world leaders in human retrovirology
  • Includes expert notes on troubleshooting and avoiding known pitfalls
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-670-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-669-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6258-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore “assay and function of accessory genes”, largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field.

Table of contents (27 chapters)

  • Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay

    Cicala, Claudia (et al.)

    Pages 3-12

  • CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α

    Zhao, Gongpu (et al.)

    Pages 13-28

  • The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating

    Yang, Yang (et al.)

    Pages 29-36

  • The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells

    Hulme, Amy E. (et al.)

    Pages 37-46

  • Imaging HIV-1 Nuclear Pre-integration Complexes

    Cereseto, Anna (et al.)

    Pages 47-54

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-670-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-669-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6258-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Human Retroviruses
Book Subtitle
Methods and Protocols
Editors
  • Elisa Vicenzi
  • Guido Poli
Series Title
Methods in Molecular Biology
Series Volume
1087
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-670-2
DOI
10.1007/978-1-62703-670-2
Hardcover ISBN
978-1-62703-669-6
Softcover ISBN
978-1-4939-6258-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVIII, 345
Number of Illustrations and Tables
24 b/w illustrations, 38 illustrations in colour
Topics