Methods in Molecular Biology

RNA Folding

Methods and Protocols

Editors: Waldsich, Christina (Ed.)

  • Includes cutting-edge methods and protocols for the study of RNA folding, which features in vivo techniques 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and vital implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-667-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-666-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6058-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Focusing on in vitro and intracellular RNA structure formation, RNA Folding: Methods and Protocols provides a comprehensive collection of experimental protocols which are suitable to dissect RNA folding pathways and to characterize the structure of RNA folding intermediates at nucleotide or even atomic resolution. The presented techniques include powerful tools with a long tradition in RNA research as well as more advanced, novel methods, thus the methods span multiple disciplines, including molecular biology, biochemistry, biophysics, and computational biology.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and authoritative, RNA Folding: Methods and Protocols serves as a vital reference for researchers attempting to gain insights into the secrets of this astounding macromolecule.

Table of contents (19 chapters)

  • Predicting RNA Structure: Advances and Limitations

    Hofacker, Ivo L. (et al.)

    Pages 1-19

  • Cis-Acting 5’ Hammerhead Ribozyme Optimization for In Vitro Transcription of Highly Structured RNAs

    Meyer, Mélanie (et al.)

    Pages 21-40

  • RNA Structural Analysis by Enzymatic Digestion

    Biondi, Elisa (et al.)

    Pages 41-52

  • The Mutate-and-Map Protocol for Inferring Base Pairs in Structured RNA

    Cordero, Pablo (et al.)

    Pages 53-77

  • Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes

    Sachsenmaier, Nora (et al.)

    Pages 79-94

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-667-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-666-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6058-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
RNA Folding
Book Subtitle
Methods and Protocols
Editors
  • Christina Waldsich
Series Title
Methods in Molecular Biology
Series Volume
1086
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-667-2
DOI
10.1007/978-1-62703-667-2
Hardcover ISBN
978-1-62703-666-5
Softcover ISBN
978-1-4939-6058-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 340
Number of Illustrations and Tables
18 b/w illustrations, 43 illustrations in colour
Topics