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Plant Metabolism

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Ganesh Sriram

    1. Engineering, University of Maryland Dept. Chemical & Biomolecular, College Park, USA

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  • Details disciplines ranging from plant biology to metabolic engineering and biotechnology
  • Provides step-by-step details essential for reproducible results
  • Contains valuable notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1083)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
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  2. Metabolites and Metabolic Fluxes

    1. Front Matter

      Pages 1-1
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    2. MeRy-B, a Metabolomic Database and Knowledge Base for Exploring Plant Primary Metabolism

      • Catherine Deborde, Daniel Jacob
      Pages 3-16

    3. Targeted Deuteration of Polyphenolics for Their Qualitative and Quantitative Metabolomic Analysis in Plant-Derived Extracts

      • Mikel R. Roe, Jerry D. Cohen, Adrian D. Hegeman
      Pages 17-29

    4. Relative Quantitation in Single-Cell Metabolomics by Laser Ablation Electrospray Mass Spectrometry

      • Bindesh Shrestha, Akos Vertes
      Pages 31-39

    5. Quantification of Plant Volatiles

      • Anthony V. Qualley, Natalia Dudareva
      Pages 41-53

    6. Quantitative Imaging Approaches for Small-Molecule Measurements Using FRET Sensors in Plants

      • Sakiko Okumoto
      Pages 55-64

    7. Isotopomer Measurement Techniques in Metabolic Flux Analysis I: Nuclear Magnetic Resonance

      • Quyen X. Truong, Jong Moon Yoon, Jacqueline V. Shanks
      Pages 65-83

    8. Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass Spectrometry

      • Jamey D. Young, Douglas K. Allen, John A. Morgan
      Pages 85-108

    9. Mathematical Modeling of Isotope Labeling Experiments for Metabolic Flux Analysis

      • Shilpa Nargund, Ganesh Sriram
      Pages 109-131

    10. Optimal Design of Isotope Labeling Experiments

      • Hong Yang, Dominic E. Mandy, Igor G. L. Libourel
      Pages 133-147

  3. Metabolic Pathways and Metabolic Systems Biology

    1. Front Matter

      Pages 149-149
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    2. Putting the Plant Metabolic Network Pathway Databases to Work: Going Offline to Gain New Capabilities

      • Kate Dreher
      Pages 151-171

    3. Elucidation of Metabolic Pathways from Enzyme Classification Data

      • Andrew G. McDonald, Keith F. Tipton
      Pages 173-186

    4. Deducing Intracellular Distributions of Metabolic Pathways from Genomic Data

      • Ansgar Gruber, Peter G. Kroth
      Pages 187-211

    5. Genome-Scale Models of Plant Metabolism

      • Margaret Simons, Ashish Misra, Ganesh Sriram
      Pages 213-230

    6. Elementary Flux Modes, Flux Balance Analysis, and Their Application to Plant Metabolism

      • Katrin Lotz, Anja Hartmann, Eva Grafahrend-Belau, Falk Schreiber, Björn H. Junker
      Pages 231-252

    7. Systems Approaches to Unraveling Plant Metabolism: Identifying Biosynthetic Genes of Secondary Metabolic Pathways

      • Martin J. Spiering, Bhavneet Kaur, James F. Parsons, Edward Eisenstein
      Pages 253-273

    8. Applications of Kinetic Modeling to Plant Metabolism

      • Johann M. Rohwer
      Pages 275-286

    9. Kinetic Modeling of Plant Metabolism and Its Predictive Power: Peppermint Essential Oil Biosynthesis as an Example

      • Bernd Markus Lange, Rigoberto Rios-Estepa
      Pages 287-311
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Keywords

About this book

In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.

Editors and Affiliations

  • Engineering, University of Maryland Dept. Chemical & Biomolecular, College Park, USA

    Ganesh Sriram

Bibliographic Information

  • Book Title: Plant Metabolism

  • Book Subtitle: Methods and Protocols

  • Editors: Ganesh Sriram

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-661-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-660-3Published: 12 November 2013

  • Softcover ISBN: 978-1-4939-5881-8Published: 17 September 2016

  • eBook ISBN: 978-1-62703-661-0Published: 11 November 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 318

  • Number of Illustrations: 36 b/w illustrations, 43 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry, Metabolomics

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