Methods in Molecular Biology

Brain Development

Methods and Protocols

Editors: Sprecher, Simon G. (Ed.)

  • Introduces cutting-edge techniques in developmental neurobiology through genetic model systems as well as some complimentary animal models
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and expert implementation advice
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-655-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-654-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6025-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes.  Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models.  Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.

Table of contents (20 chapters)

  • Immunostaining of the Developing Embryonic and Larval Drosophila Brain

    Diaper, Danielle C. (et al.)

    Pages 3-17

  • Non-fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila

    Jussen, David (et al.)

    Pages 19-35

  • Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling

    Kraft, Karoline F. (et al.)

    Pages 37-56

  • Flybow to Dissect Circuit Assembly in the Drosophila Brain

    Shimosako, Nana (et al.)

    Pages 57-69

  • Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe

    Perruchoud, Benjamin (et al.)

    Pages 71-78

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-655-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-654-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6025-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Brain Development
Book Subtitle
Methods and Protocols
Editors
  • Simon G. Sprecher
Series Title
Methods in Molecular Biology
Series Volume
1082
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-655-9
DOI
10.1007/978-1-62703-655-9
Hardcover ISBN
978-1-62703-654-2
Softcover ISBN
978-1-4939-6025-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 317
Number of Illustrations and Tables
14 b/w illustrations, 49 illustrations in colour
Topics