Methods in Molecular Biology

Fluorescence Spectroscopy and Microscopy

Methods and Protocols

Editors: Engelborghs, Yves, Visser, Antonie J.W.G. (Eds.)

  • Explores the methods and applications in the expanding fluorescence field 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
price for USA (gross)
  • ISBN 978-1-62703-649-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-62703-648-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA
  • ISBN 978-1-4939-6315-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.  The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).  Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.

 

Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Reviews

From the reviews:

“The book is illustrated with high-quality figures in both color and black and white. … This book offers techniques from internationally recognized experts. Both novice and established scientists will find it extremely useful.” (Omer Iqbal, Doody’s Book Reviews, March, 2014)

Table of contents (36 chapters)

  • How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra

    Gilmore, Adam Matthew

    Pages 3-27

  • Steady-State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions

    James, Nicholas G. (et al.)

    Pages 29-42

  • Quantitative Fluorescence Spectral Analysis of Protein Denaturation

    Stokkum, Ivo H. M. (et al.)

    Pages 43-51

  • High-Pressure Fluorescence Applications

    Dellarole, Mariano (et al.)

    Pages 53-74

  • Frequency Domain Fluorometry: Theory and Application

    Vetromile, Carissa M. (et al.)

    Pages 77-95

Buy this book

eBook $139.00
price for USA (gross)
  • ISBN 978-1-62703-649-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-62703-648-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA
  • ISBN 978-1-4939-6315-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Fluorescence Spectroscopy and Microscopy
Book Subtitle
Methods and Protocols
Editors
  • Yves Engelborghs
  • Antonie J.W.G. Visser
Series Title
Methods in Molecular Biology
Series Volume
1076
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-649-8
DOI
10.1007/978-1-62703-649-8
Hardcover ISBN
978-1-62703-648-1
Softcover ISBN
978-1-4939-6315-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XXI, 816
Number of Illustrations and Tables
73 b/w illustrations, 171 illustrations in colour
Topics