Methods in Molecular Biology

Fluorescent Protein-Based Biosensors

Methods and Protocols

Editors: Zhang, Jin, Ni, Qiang, Newman, Robert H (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-622-1
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Hardcover $119.00
price for USA
  • ISBN 978-1-62703-621-4
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Softcover $119.00
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About this book

In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction.  Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.

Table of contents (18 chapters)

  • The Design and Application of Genetically Encodable Biosensors Based on Fluorescent Proteins

    Newman, Robert H. (et al.)

    Pages 1-16

  • An Introduction to Fluorescence Imaging Techniques Geared Towards Biosensor Applications

    Goedhart, J. (et al.)

    Pages 17-28

  • Quantitative Measurement of Ca2+ and Zn2+ in Mammalian Cells Using Genetically Encoded Fluorescent Biosensors

    Park, J. Genevieve (et al.)

    Pages 29-47

  • Detecting cAMP with an Epac-Based FRET Sensor in Single Living Cells

    Klarenbeek, J. (et al.)

    Pages 49-58

  • Analysis of Compartmentalized cAMP: A Method to Compare Signals from Differently Targeted FRET Reporters

    Stangherlin, Alessandra (et al.)

    Pages 59-71

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-622-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-621-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6014-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Fluorescent Protein-Based Biosensors
Book Subtitle
Methods and Protocols
Editors
  • Jin Zhang
  • Qiang Ni
  • Robert H Newman
Series Title
Methods in Molecular Biology
Series Volume
1071
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-622-1
DOI
10.1007/978-1-62703-622-1
Hardcover ISBN
978-1-62703-621-4
Softcover ISBN
978-1-4939-6014-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 251
Number of Illustrations and Tables
12 b/w illustrations, 48 illustrations in colour
Topics