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  • © 2013

Hormone Assays in Biological Fluids

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1065)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. A Short History of Hormone Measurement

    • Michael J. Wheeler
    Pages 1-6
  3. Immunoassay Techniques

    • Michael J. Wheeler
    Pages 7-25
  4. Introduction to Gas Chromatography-Mass Spectrometry

    • Alberto Sánchez-Guijo, Michaela F. Hartmann, Stefan A. Wudy
    Pages 27-44
  5. The Measurement of LH, FSH, and Prolactin

    • Michael J. Wheeler
    Pages 105-116
  6. Measurement of Arginine Vasopressin

    • Nadia El-Farhan, David Hampton, Michael Penney
    Pages 129-139
  7. The Measurement of Anti-Müllerian Hormone (AMH)

    • Michael J. Wheeler
    Pages 141-146
  8. Measurement of Gut Hormones in Plasma

    • Steve Bloom, Mohammad Ghatei, Paul Bech
    Pages 147-170
  9. The Measurement of Androgens

    • Helen P. Field, Michael J. Wheeler
    Pages 211-226
  10. Measurement of Aldosterone in Blood

    • Sophie C. Barnes
    Pages 227-234
  11. Measurement of Plasma Renin Activity

    • Sophie C. Barnes
    Pages 235-244
  12. Measurement of Vitamin D

    • Loretta Ford
    Pages 245-257
  13. Urinary Steroid Profiling

    • Norman F. Taylor
    Pages 259-276
  14. Internal Quality Control

    • Edward Kearney
    Pages 277-289
  15. External Quality Assessment Schemes for Immunoassays

    • Catharine Sturgeon
    Pages 291-305

About this book

Hormone measurement is necessary for the diagnosis of a wide range of clinical conditions and is essential for monitoring the effectiveness of treatment. As the number of hormone requests in the clinical field rises exponentially, it has become imperative to create hormone assays accessible to researchers with a varied range of equipment. Hormone Assays in Biological Fluids, Second Edition reviews common techniques used to measure hormones as well as relatively new methods such as tandem mass spectrometry. Additionally, subsequent chapters detail methods for a broad range of hormones; Techniques and principles covered are transferable to a wide range of substances across species. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Hormone Assays in Biological Fluids, Second Edition will serve students, technologists, laboratory scientists, and researchers looking to apply, or attain a greater understanding of, methods for measuring hormones.

Editors and Affiliations

  • Consultant Clinical Scientist The Old Dairy, Exmouth, United Kingdom

    Michael J. Wheeler

Bibliographic Information

  • Book Title: Hormone Assays in Biological Fluids

  • Editors: Michael J. Wheeler

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-616-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer New York 2013

  • Hardcover ISBN: 978-1-62703-615-3Published: 01 September 2013

  • Softcover ISBN: 978-1-4939-6280-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-616-0Published: 31 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 310

  • Number of Illustrations: 20 b/w illustrations, 14 illustrations in colour

  • Topics: Molecular Medicine, Endocrinology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access