Methods in Molecular Biology

DNA Electrophoresis

Methods and Protocols

Editors: Makovets, Svetlana (Ed.)

  • Details methods that are used to study DNA using electrophoresis
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
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  • ISBN 978-1-62703-565-1
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Hardcover $119.00
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  • ISBN 978-1-62703-564-4
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Softcover $119.00
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About this book

In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.

Table of contents (19 chapters)

  • DNA Electrophoresis: Historical and Theoretical Perspectives

    Roberts, Gareth A. (et al.)

    Pages 1-9

  • Basic DNA Electrophoresis in Molecular Cloning: A Comprehensive Guide for Beginners

    Makovets, Svetlana

    Pages 11-43

  • Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-Dimensional (2D) Native–Native Agarose Gel Electrophoresis

    Robinson, Nicholas P.

    Pages 45-61

  • Analysis of DNA Structures from Eukaryotic Cells by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis

    Ivessa, Andreas S.

    Pages 63-81

  • Directionality of Replication Fork Movement Determined by Two-Dimensional Native–Native DNA Agarose Gel Electrophoresis

    Ivessa, Andreas S.

    Pages 83-103

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-565-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-564-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-5998-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
DNA Electrophoresis
Book Subtitle
Methods and Protocols
Editors
  • Svetlana Makovets
Series Title
Methods in Molecular Biology
Series Volume
1054
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, New York
eBook ISBN
978-1-62703-565-1
DOI
10.1007/978-1-62703-565-1
Hardcover ISBN
978-1-62703-564-4
Softcover ISBN
978-1-4939-5998-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 299
Number of Illustrations and Tables
36 b/w illustrations, 20 illustrations in colour
Topics