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Immobilization of Enzymes and Cells

  • Book
  • © 2013

Overview

Editors:
  1. Jose M. Guisan

    1. Instituto de Catlisis y Petroleoquimica, CSIC, Madrid, Spain

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  • Demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1051)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. New Opportunities for Immobilization of Enzymes

    • Jose M. Guisan
    Pages 1-13

  3. Immobilization of Enzymes: A Literature Survey

    • Beatriz Brena, Paula González-Pombo, Francisco Batista-Viera
    Pages 15-31

  4. Glutaraldehyde-Mediated Protein Immobilization

    • Fernando López-Gallego, Jose M. Guisán, Lorena Betancor
    Pages 33-41

  5. Immobilization of Enzymes on Monofunctional and Heterofunctional Epoxy-Activated Supports

    • Cesar Mateo, Valeria Grazu, Jose M. Guisan
    Pages 43-57

  6. Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Groups

    • Fernando López-Gallego, Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Cesar Mateo, Jose M. Guisan
    Pages 59-71

  7. Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports

    • Cesar Mateo, Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Jose M. Guisan
    Pages 73-88

  8. Reversible Covalent Immobilization of Enzymes via Disulfide Bonds

    • Karen Ovsejevi, Carmen Manta, Francisco Batista-Viera
    Pages 89-116

  9. Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in Low-Water Media

    • Joyeeta Mukherjee, Kusum Solanki, Munishwar Nath Gupta
    Pages 117-127

  10. Immobilization of Enzymes by Bioaffinity Layering

    • Veena Singh, Meryam Sardar, Munishwar Nath Gupta
    Pages 129-137

  11. Immobilization of Enzymes on Magnetic Beads Through Affinity Interactions

    • Audrey Sassolas, Akhtar Hayat, Jean-Louis Marty
    Pages 139-148

  12. Tips for the Functionalization of Nanoparticles with Antibodies

    • Ester Polo, Sara Puertas, María Moros, Pilar Batalla, José M. Guisán, Jesús M. de la Fuente et al.
    Pages 149-163

  13. Design and Characterization of Functional Nanoparticles for Enhanced Bio-performance

    • Pablo del Pino, Scott G. Mitchell, Beatriz Pelaz
    Pages 165-207

  14. Immobilization of Enzymes on Ethynyl-Modified Electrodes via Click Chemistry

    • Akhtar Hayat, Audrey Sassolas, Amina Rhouati, Jean-Louis Marty
    Pages 209-216

  15. Modification of Carbon Nanotube Electrodes with 1-Pyrenebutanoic Acid, Succinimidyl Ester for Enhanced Bioelectrocatalysis

    • Guinevere Strack, Robert Nichols, Plamen Atanassov, Heather R. Luckarift, Glenn R. Johnson
    Pages 217-228

  16. Enzyme Immobilization by Entrapment Within a Gel Network

    • Audrey Sassolas, Akhtar Hayat, Jean-Louis Marty
    Pages 229-239

  17. Practical Protocols for Lipase Immobilization via Sol–Gel Techniques

    • Manfred T. Reetz
    Pages 241-254

  18. Improving Lipase Activity by Immobilization and Post-immobilization Strategies

    • Jose M. Palomo, Marco Filice, Oscar Romero, Jose M. Guisan
    Pages 255-273

  19. High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic Solvents and Ionic Liquids

    • Ipsita Roy, Joyeeta Mukherjee, Munishwar Nath Gupta
    Pages 275-284

  20. Biomedical Applications of Immobilized Enzymes: An Update

    • Marta Pastor, Amaia Esquisabel, José Luis Pedraz
    Pages 285-299
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Keywords

About this book

For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols,  in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

Editors and Affiliations

  • Instituto de Catlisis y Petroleoquimica, CSIC, Madrid, Spain

    Jose M. Guisan

Bibliographic Information

  • Book Title: Immobilization of Enzymes and Cells

  • Editors: Jose M. Guisan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-550-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-549-1Published: 11 August 2013

  • Softcover ISBN: 978-1-4939-5988-4Published: 27 August 2016

  • eBook ISBN: 978-1-62703-550-7Published: 13 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 377

  • Number of Illustrations: 77 b/w illustrations, 39 illustrations in colour

  • Topics: Cell Biology, Enzymology

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