Methods in Molecular Biology

G Protein-Coupled Receptor Signaling in Plants

Methods and Protocols

Editors: Running, Mark P. (Ed.)

  • Explores techniques to examine the roles of GCRPs and small GTPases in key plant biological processes
  • Features step-by-step protocols that are ready for the lab
  • Includes expert tips and key implementation advice to ensure quality results
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-532-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-531-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6216-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins.  Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.

Table of contents (16 chapters)

  • Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs

    Gookin, Timothy E. (et al.)

    Pages 1-12

  • Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins

    Choudhury, Swarup Roy (et al.)

    Pages 13-20

  • Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana

    Zhao, Jian (et al.)

    Pages 21-35

  • Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants

    Jin, Zhaoqing (et al.)

    Pages 37-43

  • Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G-Proteins in Arabidopsis

    Tanaka, Kiwamu (et al.)

    Pages 45-54

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-532-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-531-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6216-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
G Protein-Coupled Receptor Signaling in Plants
Book Subtitle
Methods and Protocols
Editors
  • Mark P. Running
Series Title
Methods in Molecular Biology
Series Volume
1043
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-532-3
DOI
10.1007/978-1-62703-532-3
Hardcover ISBN
978-1-62703-531-6
Softcover ISBN
978-1-4939-6216-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 162
Number of Illustrations and Tables
5 b/w illustrations, 14 illustrations in colour
Topics