Methods in Molecular Biology

The Inflammasome

Methods and Protocols

Editors: De Nardo, Christine M., Latz, Eicke (Eds.)

  • Presents current up-to-date methodologies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-523-1
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About this book

 Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1β cytokines, and analysis of inflammasome assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Table of contents (16 chapters)

  • Assessing β-Amyloid-Induced NLRP3 Inflammasome Activation in Primary Microglia

    Schnaars, Mareike (et al.)

    Pages 1-8

  • Activating the NLRP3 Inflammasome Using the Amyloidogenic Peptide IAPP

    Westwell-Roper, Clara (et al.)

    Pages 9-18

  • Assessment and Quantification of Crystal-Induced Lysosomal Damage

    Duewell, Peter (et al.)

    Pages 19-27

  • Assessment of Inflammasome Activation in Primary Human Immune Cells

    Plantinga, Theo S. (et al.)

    Pages 29-39

  • NLRP3 Inflammasome Activation and Cytotoxicity Induced by Particulate Adjuvants

    Yang, Marie (et al.)

    Pages 41-63

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-523-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-522-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6011-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Inflammasome
Book Subtitle
Methods and Protocols
Editors
  • Christine M. De Nardo
  • Eicke Latz
Series Title
Methods in Molecular Biology
Series Volume
1040
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-523-1
DOI
10.1007/978-1-62703-523-1
Hardcover ISBN
978-1-62703-522-4
Softcover ISBN
978-1-4939-6011-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 225
Number of Illustrations and Tables
23 b/w illustrations, 21 illustrations in colour
Topics