Methods in Molecular Biology

Deep Sequencing Data Analysis

Editors: Shomron, Noam (Ed.)

  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-62703-514-9
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About this book

The new genetic revolution is fuelled by Deep Sequencing (or Next Generation Sequencing) apparatuses which, in essence, read billions of nucleotides per reaction. Effectively, when carefully planned, any experimental question which can be translated into reading nucleic acids can be applied.In Deep Sequencing Data Analysis, expert researchers in the field detail methods which are now commonly used to study the multi-facet deep sequencing data field. These included techniques for compressing of data generated, Chromatin Immunoprecipitation (ChIP-seq), and various approaches for the identification of sequence variants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Deep Sequencing Data Analysis seeks to aid scientists in the further understanding of key data analysis procedures for deep sequencing data interpretation.

Table of contents (13 chapters)

  • An Introduction to High-Throughput Sequencing Experiments: Design and Bioinformatics Analysis

    Normand, Rachelly (et al.)

    Pages 1-26

  • Compressing Resequencing Data with GReEn

    Pinho, Armando J. (et al.)

    Pages 27-37

  • On the Accuracy of Short Read Mapping

    Menzel, Peter (et al.)

    Pages 39-59

  • Statistical Modeling of Coverage in High-Throughput Data

    Golan, David (et al.)

    Pages 61-79

  • Assembly Algorithms for Deep Sequencing Data: Basics and Pitfalls

    Kol, Nitzan (et al.)

    Pages 81-91

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-514-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-513-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6027-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Deep Sequencing Data Analysis
Editors
  • Noam Shomron
Series Title
Methods in Molecular Biology
Series Volume
1038
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-514-9
DOI
10.1007/978-1-62703-514-9
Hardcover ISBN
978-1-62703-513-2
Softcover ISBN
978-1-4939-6027-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 234
Number of Illustrations and Tables
38 b/w illustrations, 43 illustrations in colour
Topics