Methods in Molecular Biology

Stem Cell Niche

Methods and Protocols

Editors: Turksen, Kursad (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-508-8
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Hardcover $119.00
price for USA
  • ISBN 978-1-62703-507-1
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Softcover $119.00
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About this book

Interest in a specialized microenvironment or “niche” regulating hemopoietic stem cell function has been steadily growing since the idea was first proposed by Ray Schofield over three decades ago. This growing interest, as well as more recently the interest in cellular-molecular-biochemical characterization of not only the hemopoietic stem cell niche but the niches for other stem cells, incited the compilation of Stem Cell Niche: Methods and Protocols. In this volume, scientists have provided protocols that will provide both a flavor of the field and hopefully stimulate new approaches and methodologies by those interested in the stem cell niche. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Stem Cell Niche: Methods and Protocols seeks to serve both experts and novices in the stem cell field with well-established protocols on this exciting subject.  

Table of contents (21 chapters)

  • Immunostaining of Germline Stem Cells and the Niche in Drosophila Ovaries

    Luo, Lichao (et al.)

    Pages 1-7

  • Genetic, Immunofluorescence Labeling, and In Situ Hybridization Techniques in Identification of Stem Cells in Male and Female Germline Niches

    Singh, Shree Ram (et al.)

    Pages 9-23

  • Visualization of Adult Stem Cells Within Their Niches Using the Drosophila Germline as a Model System

    König, Annekatrin (et al.)

    Pages 25-33

  • Morphometric Evaluation of the Spermatogonial Stem Cell Distribution and Niche in Vertebrates

    Campos-Junior, Paulo Henrique Almeida (et al.)

    Pages 35-42

  • In Vitro Construction of 2D and 3D Simulations of the Murine Hematopoietic Niche

    Chitteti, Brahmananda Reddy (et al.)

    Pages 43-56

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-508-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-507-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA
  • ISBN 978-1-4939-6037-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Stem Cell Niche
Book Subtitle
Methods and Protocols
Editors
  • Kursad Turksen
Series Title
Methods in Molecular Biology
Series Volume
1035
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-508-8
DOI
10.1007/978-1-62703-508-8
Hardcover ISBN
978-1-62703-507-1
Softcover ISBN
978-1-4939-6037-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 270
Number of Illustrations and Tables
21 b/w illustrations, 53 illustrations in colour
Topics