Methods in Molecular Biology

Mouse Models of Innate Immunity

Methods and Protocols

Editors: Allen, Irving C. (Ed.)

  • Includes detailed contributions from a diverse group of authors
  • Features both ex vivo and in vivo protocols that are lab-ready
  • Contains expert tips and key implementation advice to ensure successful results
see more benefits

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-481-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-480-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover n/a
  • ISBN 978-1-4939-6036-1
  • Free shipping for individuals worldwide
About this book

The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.

Table of contents (24 chapters)

  • Conventional Murine Gene Targeting

    Zimmermann, Albert G. (et al.)

    Pages 1-18

  • Production and Characterization of Humanized Rag2−/−γc−/− Mice

    Sanchez, Freddy M. (et al.)

    Pages 19-26

  • Isolation, Culture, and Functional Evaluation of Bone Marrow-Derived Macrophages

    Davis, Beckley K.

    Pages 27-35

  • Collecting Resident or Thioglycollate-Elicited Peritoneal Macrophages

    Schneider, Monika

    Pages 37-40

  • Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils

    Vong, Linda (et al.)

    Pages 41-50

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-62703-481-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-480-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover n/a
  • ISBN 978-1-4939-6036-1
  • Free shipping for individuals worldwide
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Mouse Models of Innate Immunity
Book Subtitle
Methods and Protocols
Editors
  • Irving C. Allen
Series Title
Methods in Molecular Biology
Series Volume
1031
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-481-4
DOI
10.1007/978-1-62703-481-4
Hardcover ISBN
978-1-62703-480-7
Softcover ISBN
978-1-4939-6036-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 225
Number of Illustrations and Tables
7 b/w illustrations, 20 illustrations in colour
Topics