Methods in Molecular Biology

Glycosyltransferases

Methods and Protocols

Editors: Brockhausen, Inka (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-465-4
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  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-464-7
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Softcover $169.00
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About this book

Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease.  Complex glycoconjugates, such as glycoproteins, proteoglycans and glycolipids, are assembled by GTs which synthesize specific linkages between sugars or sugars and protein. This is in contrast to the non-specific or less specific chemical glycation reactions, transglycosylation and  reverse glycosylation reactions. Glycosyltransferases: Methods and Protocols contains a wide range of studies, methods and protocols which form a solid basis for investigations of the role and mechanisms, biology and pathology involving GTs. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Glycosyltransferases: Methods and Protocols is a vital contribution to glycobiology and glycopathology, and to applications of these enzymes in biotechnology and drug development. It will prove invaluable to students, postdoctoral fellows, and senior scientists carrying on research of GTs that has been intensified over the last years.

Table of contents (31 chapters)

  • Glycan Microarray Screening Assay for Glycosyltransferase Specificities

    Peng, Wenjie (et al.)

    Pages 1-14

  • A Fluorescence-Based Assay for Core 1 β3Galactosyltransferase (T-Synthase) Activity

    Ju, Tongzhong (et al.)

    Pages 15-28

  • Structural and Biochemical Analysis of a Bacterial Glycosyltransferase

    Zhu, Fan (et al.)

    Pages 29-39

  • Study of the Biological Functions of Mucin Type Core 3 O-glycans

    Lee, Seung Ho (et al.)

    Pages 41-50

  • Generation of Anti-sulfated Glycan Antibodies Using Sulfotransferase-Deficient Mice

    Kawashima, Hiroto

    Pages 51-60

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-465-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-464-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-4939-5996-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Glycosyltransferases
Book Subtitle
Methods and Protocols
Editors
  • Inka Brockhausen
Series Title
Methods in Molecular Biology
Series Volume
1022
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-465-4
DOI
10.1007/978-1-62703-465-4
Hardcover ISBN
978-1-62703-464-7
Softcover ISBN
978-1-4939-5996-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVII, 425
Number of Illustrations and Tables
70 b/w illustrations, 40 illustrations in colour
Topics