Methods in Molecular Biology

Tandem Repeats in Genes, Proteins, and Disease

Methods and Protocols

Editors: Hatters, Danny M., Hannan, Anthony J. (Eds.)

  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-438-8
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  • Immediate eBook download after purchase
Hardcover $139.00
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  • ISBN 978-1-62703-437-1
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Softcover $139.00
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About this book

The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences.  Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine.  Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states.  This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular  and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.

Table of contents (18 chapters)

  • Longitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded Proteins

    Tsvetkov, Andrey S. (et al.)

    Pages 1-20

  • Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces

    Burke, Kathleen A. (et al.)

    Pages 21-40

  • Morphometric Analysis of Huntington’s Disease Neurodegeneration in Drosophila

    Song, Wan (et al.)

    Pages 41-57

  • Size Analysis of Polyglutamine Protein Aggregates Using Fluorescence Detection in an Analytical Ultracentrifuge

    Polling, Saskia (et al.)

    Pages 59-71

  • A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins

    Robertson, Amy L. (et al.)

    Pages 73-83

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-438-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-437-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-6300-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Tandem Repeats in Genes, Proteins, and Disease
Book Subtitle
Methods and Protocols
Editors
  • Danny M. Hatters
  • Anthony J. Hannan
Series Title
Methods in Molecular Biology
Series Volume
1017
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-438-8
DOI
10.1007/978-1-62703-438-8
Hardcover ISBN
978-1-62703-437-1
Softcover ISBN
978-1-4939-6300-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 258
Number of Illustrations and Tables
32 b/w illustrations, 23 illustrations in colour
Topics