Methods in Molecular Biology

Corneal Regenerative Medicine

Methods and Protocols

Editors: Wright, Bernice, Connon, Che J (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-432-6
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Hardcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-62703-431-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-6272-3
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  • Usually dispatched within 3 to 5 business days.
About this book

Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of corneal equivalents as replacement tissue is underway to develop viable corneal prosthetics. Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine. Divided into four convenient sections, topics include the identification, characterisation and cultivation of LESC, as well as the investigation of biopolymers used as the basis for corneal substitutes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Corneal Regenerative Medicine: Methods and Protocols covers the fundamental techniques useful for both the laboratory and clinical settings.  

Table of contents (15 chapters)

  • Limbal Epithelial Cell Therapy: Past, Present, and Future

    Utheim, Tor Paaske

    Pages 3-43

  • The Artificial Cornea

    Polisetti, Naresh (et al.)

    Pages 45-52

  • Clonal Analysis of Limbal Epithelial Stem Cell Populations

    Schlötzer-Schrehardt, Ursula

    Pages 55-64

  • The Use of Bromodeoxyuridine Incorporation Assays to Assess Corneal Stem Cell Proliferation

    Crane, Ashley M. (et al.)

    Pages 65-70

  • Enrichment of Human Corneal Epithelial Stem/Progenitor Cells by Magnetic Bead Sorting Using SSEA4 as a Negative Marker

    Nakatsu, Martin N. (et al.)

    Pages 71-77

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-432-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-62703-431-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-6272-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Corneal Regenerative Medicine
Book Subtitle
Methods and Protocols
Editors
  • Bernice Wright
  • Che J Connon
Series Title
Methods in Molecular Biology
Series Volume
1014
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-432-6
DOI
10.1007/978-1-62703-432-6
Hardcover ISBN
978-1-62703-431-9
Softcover ISBN
978-1-4939-6272-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 230
Number of Illustrations and Tables
23 b/w illustrations, 33 illustrations in colour
Topics