Methods in Molecular Biology

Vascular Proteomics

Methods and Protocols

Editors: Vivanco, Fernando (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

Proteomics is a rapidly expanding investigation platform in cardiovascular medicine. Driven by major improvements in mass spectrometry (MS) instrumentation and data analysis, the proteomics field has flourished in recent years particularly in the study of complex diseases. These recent advances are characterized by the development of quantitative MS-based methods that promoted the field from primarily identifying proteins to also providing measurements of relative changes in protein levels between different cell states. This progress is reflected in the application of proteomic techniques to vascular pathology. Vascular Proteomics: Methods and Protocols provides up-to-date methods and protocols for the analysis of arteries, cells, lipoproteins, body fluids, and metabolites, with a particular focus on MS-based methods of protein and peptide quantification. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Vascular Proteomics: Methods and Protocols is a representative selection of methods that can be a useful resource for experienced proteomics practitioners as well as newcomers interested in becoming acquainted with the practice of proteomic techniques for cardiovascular research.

Table of contents (16 chapters)

  • Vascular Proteomics

    Barderas, Maria G. (et al.)

    Pages 1-20

  • Laser Microdissection and Saturation Labeling DIGE Method for the Analysis of Human Arteries

    Cuesta, Fernando (et al.)

    Pages 21-32

  • Use of TOF-SIMS in Vascular Biology

    Mas, Sebastián (et al.)

    Pages 33-43

  • Proteomic Analysis of Vascular Smooth Muscle Cells with S- and R-Enantiomers of Atenolol by iTRAQ and LC-MS/MS

    Zhang, Jianhua (et al.)

    Pages 45-52

  • Phosphoproteomic Analysis of Aortic Endothelial Cells Activated by Oxidized Phospholipids

    Zimman, Alejandro (et al.)

    Pages 53-69

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-405-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA
  • ISBN 978-1-62703-404-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Vascular Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Fernando Vivanco
Series Title
Methods in Molecular Biology
Series Volume
1000
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-405-0
DOI
10.1007/978-1-62703-405-0
Hardcover ISBN
978-1-62703-404-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 230
Topics