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Plant Lipid Signaling Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Teun Munnik

    1. Swammerdam Inst. Life Science, Sect. Plant Pathology, University of Amsterdam, Amsterdam, The Netherlands

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  2. Ingo Heilmann

    1. Institute for Biochemistry and Biotechno, Department of Cellular Biochemistry, Martin-Luther-Univ Halle-Wittenberg, Halle, Germany

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  • Presents timely and important tools needed to elucidate particular roles of lipid (-derived) signals in plants
  • Includes easy-to-use, reproducible protocols
  • Contains expert tips on troubleshooting and avoiding known pitfalls
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1009)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Lipid Analyses

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Analyzing Plant Signaling Phospholipids Through 32Pi-Labeling and TLC

      • Teun Munnik, Xavier Zarza
      Pages 3-15

    3. Analysis of D3-,4-,5-Phosphorylated Phosphoinositides Using HPLC

      • Teun Munnik
      Pages 17-24

    4. Mass Measurement of Polyphosphoinositides by Thin-Layer and Gas Chromatography

      • Mareike Heilmann, Ingo Heilmann
      Pages 25-32

    5. Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay

      • Ingo Heilmann, Imara Y. Perera
      Pages 33-41

    6. Quantification of Diacylglycerol by Mass Spectrometry

      • Katharina vom Dorp, Isabel Dombrink, Peter Dörmann
      Pages 43-54

    7. Distinguishing Phosphatidic Acid Pools from De Novo Synthesis, PLD, and DGK

      • Steven A. Arisz, Teun Munnik
      Pages 55-62

    8. Use of Phospholipase A2 for the Production of Lysophospholipids

      • Steven A. Arisz, Teun Munnik
      Pages 63-68

    9. Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas Chromatography

      • Vera Wewer, Peter Dörmann, Georg Hölzl
      Pages 69-78

    10. Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry

      • Sunitha Shiva, Hieu Sy Vu, Mary R. Roth, Zhenguo Zhou, Shantan Reddy Marepally, Daya Sagar Nune et al.
      Pages 79-91

    11. Detection and Quantification of Plant Sphingolipids by LC-MS

      • Jennifer E. Markham
      Pages 93-101

    12. Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra-performance Liquid Chromatography/Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid

      • Nadja Stingl, Markus Krischke, Agnes Fekete, Martin J. Mueller
      Pages 103-113

    13. Analysis of Fatty Acid Amide Hydrolase Activity in Plants

      • Sang-Chul Kim, Lionel Faure, Kent D. Chapman
      Pages 115-127

    14. Ionization Behavior of Polyphosphoinositides Determined via the Preparation of pH Titration Curves Using Solid-State 31P NMR

      • Zachary T. Graber, Edgar E. Kooijman
      Pages 129-142

  3. Enzyme Activities

    1. Front Matter

      Pages 143-143
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    2. Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli- Expressed Recombinant Peptides

      • Sylvie Collin, Françoise Cochet
      Pages 145-161

    3. Phosphatidylinositol 4-Kinase and Phosphatidylinositol 4-Phosphate 5-Kinase Assays

      • Yang Ju Im, Irena Brglez, Catherine Dieck, Imara Y. Perera, Wendy F. Boss
      Pages 163-174

    4. Assaying Inositol and Phosphoinositide Phosphatase Enzymes

      • Janet L. Donahue, Mustafa Ercetin, Glenda E. Gillaspy
      Pages 175-185

    5. Determination of Phospholipase C Activity In Vitro

      • S. M. Teresa Hernández-Sotomayor, J. Armando Muñoz-Sanchez
      Pages 187-192
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Keywords

About this book

As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In  Plant Lipid Signaling Protocols,  expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied.  These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.

Editors and Affiliations

  • Swammerdam Inst. Life Science, Sect. Plant Pathology, University of Amsterdam, Amsterdam, The Netherlands

    Teun Munnik

  • Institute for Biochemistry and Biotechno, Department of Cellular Biochemistry, Martin-Luther-Univ Halle-Wittenberg, Halle, Germany

    Ingo Heilmann

Bibliographic Information

  • Book Title: Plant Lipid Signaling Protocols

  • Editors: Teun Munnik, Ingo Heilmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-401-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-400-5Published: 17 May 2013

  • Softcover ISBN: 978-1-4939-5927-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-401-2Published: 17 May 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 305

  • Number of Illustrations: 22 b/w illustrations, 15 illustrations in colour

  • Topics: Plant Sciences

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