Methods in Molecular Biology

Plant Lipid Signaling Protocols

Editors: Munnik, Teun, Heilmann, Ingo (Eds.)

  • Presents timely and important tools needed to elucidate particular roles of  lipid (-derived) signals in plants
  • Includes easy-to-use, reproducible protocols
  • Contains expert tipes on troubleshooting and avoiding known pitfalls
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-401-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-400-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5927-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In  Plant Lipid Signaling Protocols,  expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied.  These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.

Table of contents (27 chapters)

  • Analyzing Plant Signaling Phospholipids Through 32Pi-Labeling and TLC

    Munnik, Teun (et al.)

    Pages 3-15

  • Analysis of D3-,4-,5-Phosphorylated Phosphoinositides Using HPLC

    Munnik, Teun

    Pages 17-24

  • Mass Measurement of Polyphosphoinositides by Thin-Layer and Gas Chromatography

    Heilmann, Mareike (et al.)

    Pages 25-32

  • Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay

    Heilmann, Ingo (et al.)

    Pages 33-41

  • Quantification of Diacylglycerol by Mass Spectrometry

    Dorp, Katharina (et al.)

    Pages 43-54

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-401-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-400-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5927-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Lipid Signaling Protocols
Editors
  • Teun Munnik
  • Ingo Heilmann
Series Title
Methods in Molecular Biology
Series Volume
1009
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
Distribution Rights
Distribution rights for India: Mehul Book Sales, Mumbai, India
eBook ISBN
978-1-62703-401-2
DOI
10.1007/978-1-62703-401-2
Hardcover ISBN
978-1-62703-400-5
Softcover ISBN
978-1-4939-5927-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 305
Number of Illustrations and Tables
22 b/w illustrations, 15 illustrations in colour
Topics