Methods in Molecular Biology

Protein-Ligand Interactions

Methods and Applications

Editors: Williams, Mark, Daviter, Tina (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-62703-398-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-62703-397-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-4939-5873-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions – whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology.

Table of contents (19 chapters)

  • Protein–Ligand Interactions: Fundamentals

    Williams, Mark A.

    Pages 3-34

  • Protein Sample Characterization

    Daviter, Tina (et al.)

    Pages 35-62

  • Measurement of Protein–Ligand Complex Formation

    Lowe, Peter N. (et al.)

    Pages 63-99

  • Isothermal Titration Calorimetry for Studying Protein–Ligand Interactions

    Damian, Luminita

    Pages 103-118

  • Rapid Mixing Kinetic Techniques

    Martin, Stephen R. (et al.)

    Pages 119-138

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-62703-398-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-62703-397-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-4939-5873-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Protein-Ligand Interactions
Book Subtitle
Methods and Applications
Editors
  • Mark Williams
  • Tina Daviter
Series Title
Methods in Molecular Biology
Series Volume
1008
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-398-5
DOI
10.1007/978-1-62703-398-5
Hardcover ISBN
978-1-62703-397-8
Softcover ISBN
978-1-4939-5873-3
Series ISSN
1064-3745
Edition Number
2
Number of Pages
X, 530
Number of Illustrations and Tables
101 b/w illustrations, 45 illustrations in colour
Topics