Methods in Molecular Biology

Microsatellites

Methods and Protocols

Editors: Kantartzi, Stella K. (Ed.)

  • Includes cutting-edge methods and protocols for microsatellite markers in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-62703-389-3
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About this book

Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems.  Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research.  Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.

Table of contents (21 chapters)

  • Microsatellites: Evolution and Contribution

    Madesis, Panagiotis (et al.)

    Pages 1-13

  • Screening of Genomic Libraries

    Novelli, Valdenice M. (et al.)

    Pages 17-24

  • PCR-Based Isolation of Microsatellite Arrays (PIMA)

    Lin, Heng-Sheng (et al.)

    Pages 25-55

  • Fast Isolation by AFLP of Sequences Containing Repeats

    Rikalainen, Kaisa

    Pages 57-66

  • Microsatellite DNA Capture from Enriched Libraries

    Gonzalez, Elena G. (et al.)

    Pages 67-87

Buy this book

eBook n/a
  • ISBN 978-1-62703-389-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-62703-388-6
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Microsatellites
Book Subtitle
Methods and Protocols
Editors
  • Stella K. Kantartzi
Series Title
Methods in Molecular Biology
Series Volume
1006
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-389-3
DOI
10.1007/978-1-62703-389-3
Hardcover ISBN
978-1-62703-388-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 339
Topics