Skip to main content
  • Book
  • © 2013

Heart Proteomics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1005)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
  2. Large-Scale Characterization of the Murine Cardiac Proteome

    • Jake Cosme, Andrew Emili, Anthony O. Gramolini
    Pages 1-10
  3. Determining Protein Concentrations of the Human Ventricular Proteome

    • Arjen Scholten, Albert J. R. Heck
    Pages 11-24
  4. Multidimensional Protein Identification Technology for Direct-Tissue Proteomics of Heart

    • Dario Di Silvestre, Francesca Brambilla, Pier Luigi Mauri
    Pages 25-38
  5. Global Protein Quantification of Mouse Heart Tissue Based on the SILAC Mouse

    • Anne Konzer, Aaron Ruhs, Thomas Braun, Marcus Krüger
    Pages 39-52
  6. Global Proteomic Profiling and Enrichment Maps of Dilated Cardiomyopathy

    • Ruth Isserlin, Daniele Merico, Andrew Emili
    Pages 53-66
  7. Systems Proteomics of Healthy and Diseased Chromatin

    • Haodong Chen, Emma Monte, Thomas M. Vondriska, Sarah Franklin
    Pages 77-93
  8. Proteomic Analysis of Interstitial Aortic Valve Cells Acquiring a Pro-calcific Profile

    • Millioni Renato, Elisa Bertacco, Cinzia Franchin, Giorgio Arrigoni, Marcello Rattazzi
    Pages 95-107
  9. Differential Protein Expression Analysis of Degenerative Aortic Stenosis by iTRAQ Labeling

    • Sergio Alonso-Orgaz, Tatiana Martin-Rojas, Enrique Calvo, Juan Antonio López, Fernando Vivanco, María G. Barderas
    Pages 109-117
  10. Characterization of Zebrafish Cardiac Proteome Using Online pH Gradient SCX–RP HPLC–MS/MS Platform

    • Jiang Zhang, Kevin A. Lanham, Warren Heideman, Richard E. Peterson, Lingjun Li
    Pages 119-127
  11. Proteomic Analysis of Brain Mitochondrial Proteome and Mitochondrial Complexes

    • Ana Lopez-Campistrous, Carlos Fernandez-Patron
    Pages 129-141
  12. Oxidative Modifications of Mitochondria Complex II

    • Liwen Zhang, Patrick T. Kang, Chwen-Lih Chen, Kari B. Green, Yeong-Renn Chen
    Pages 143-156
  13. Detection of O-GlcNAc Modifications on Cardiac Myofilament Proteins

    • Genaro A. Ramirez-Correa, Isabel Martinez Ferrando, Gerald Hart, Anne Murphy
    Pages 157-168
  14. Quantification of Mitochondrial S-Nitrosylation by CysTMT6 Switch Assay

    • Christopher I. Murray, Hea Seung Chung, Helge Uhrigshardt, Jennifer E. Van Eyk
    Pages 169-179
  15. Identification of Thioredoxin Target Protein Networks in Cardiac Tissues of a Transgenic Mouse

    • Cexiong Fu, Tong Liu, Andrew M. Parrott, Hong Li
    Pages 181-197
  16. A Sequential Extraction Methodology for Cardiac Extracellular Matrix Prior to Proteomics Analysis

    • Javier Barallobre-Barreiro, Athanasios Didangelos, Xiaoke Yin, Nieves Doménech, Manuel Mayr
    Pages 215-223
  17. Optimized Method for Identification of the Proteomes Secreted by Cardiac Cells

    • Miroslava Stastna, Jennifer E. Van Eyk
    Pages 225-235
  18. Secretome of Human Aortic Valves

    • Fernando de la Cuesta, Gloria Alvarez-Llamas, Felix Gil-Dones, Verónica M. Darde, Enrique Calvo, Juan Antonio López et al.
    Pages 237-243

About this book

The use of proteomics to study complex diseases such as cardiovascular disease, the leading cause of death in developed countries, has grown exponentially in recent years. Proteomics is a rapidly expanding investigation platform in cardiovascular medicine and is becoming integrated and incorporated into cardiovascular research. The proteomics field continues to develop with major improvements in mass spectrometry instrumentation, methodology and data analysis. Heart Proteomics: Methods and Protocols complies a selection of techniques and methods that target the numerous processes implicated in the pathophysiology of heart. Chapters cover protocols and updated methods in the heart proteomic area with a particular focus on MS-based methods of protein and peptide quantification and the analysis of posttranslational modifications as well as descriptions of system biology approaches, which provide a better understanding of normal and pathological processes. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Heart Proteomics: Methods and Protocols is a representative selection of methods that will prove to be a useful resource for experienced proteomics practitioners and newcomers alike.

Editors and Affiliations

  • Depto. Immunología, Fundación Jiménez Díaz, Madrid, Spain

    Fernando Vivanco

Bibliographic Information

  • Book Title: Heart Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Fernando Vivanco

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-386-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-385-5Published: 20 April 2013

  • Softcover ISBN: 978-1-4939-6000-2Published: 23 August 2016

  • eBook ISBN: 978-1-62703-386-2Published: 20 April 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 262

  • Number of Illustrations: 16 b/w illustrations, 31 illustrations in colour

  • Topics: Molecular Medicine, Cardiology, Proteomics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access