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  • Book
  • © 2013

Organ Regeneration

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1001)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiii
  2. Isolation of Pulsatile Cell Bodies from Esophageal Tissue

    • John W. Ludlow, Joydeep Basu, Christopher W. Genheimer, Kelly I. Guthrie, Namrata Sangha
    Pages 35-42
  3. Ex Vivo Culture and Separation of Functional Renal Cells

    • Andrew T. Bruce, Kelly I. Guthrie, Rusty Kelley
    Pages 53-64
  4. Isolation and Myogenic Differentiation of Mesenchymal Stem Cells for Urologic Tissue Engineering

    • Rongpei Wu, Guihua Liu, Shantaram Bharadwaj, Yuanyuan Zhang
    Pages 65-80
  5. Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine

    • Teresa Bora Burnette, Andrew T. Bruce
    Pages 115-132
  6. Preparation and Evaluation of Natural Scaffold Materials for Kidney Regenerative Applications

    • Monica A. Serban, Toyin A. Knight, Richard G. Payne
    Pages 133-143
  7. Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration

    • Adelola O. Oseni, Peter E. Butler, Alexander M. Seifalian
    Pages 145-152
  8. Migration Assay to Evaluate Cellular Interactions with Biomaterials for Tissue Engineering/Regenerative Medicine Applications

    • Kelly I. Guthrie, Namrata Sangha, Christopher W. Genheimer, Joydeep Basu, John W. Ludlow
    Pages 189-196
  9. Bioreactor Design Considerations for Hollow Organs

    • Jeff Fish, Craig Halberstadt, Darell W. McCoy, Neil Robbins
    Pages 207-214
  10. Construction of a Multicoaxial Hollow Fiber Bioreactor

    • Randall McClelland, Katherine Tech, Jeffrey M. Macdonald
    Pages 215-226

About this book

Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. Organ Regeneration: Methods and Protocols has been assembled in response to the growing interest in organ and tissue regeneration as a means to treat disease. Topics cover methods such as isolation and characterization of cells from selected soft tissues and solid organs, preparation and evaluation of natural and synthetic biomaterial scaffolding, implantation of regenerative constructs within experimental animals, and evaluation of regenerative outcomes by molecular and histological methodologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Organ Regeneration: Methods and Protocols serves as a detailed guide to aid newcomers and seasoned veterans in their developmental and experimental work in tissue engineering and regenerative medicine.

Editors and Affiliations

  • Tengion, Inc, Winston-Salem, USA

    Joydeep Basu

  • Zen Bio, Inc., Research Triangle Park, USA

    John W. Ludlow

Bibliographic Information

  • Book Title: Organ Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Joydeep Basu, John W. Ludlow

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-363-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-362-6Published: 14 March 2013

  • Softcover ISBN: 978-1-4939-6008-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-363-3Published: 15 March 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 377

  • Topics: Molecular Medicine, Regenerative Medicine/Tissue Engineering

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access