Methods in Molecular Biology

Proteomics for Biomarker Discovery

Editors: Zhou, Ming, Veenstra, Timothy (Eds.)

  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-360-2
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  • Immediate eBook download after purchase
Hardcover $139.00
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  • ISBN 978-1-62703-359-6
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Softcover $139.00
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About this book

With the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures.  While the mass spectrometer resides at the heart of proteomics, its ability to characterize biological samples is only as good as the sample preparation and data analysis tools used in any study.  In Proteomics for Biomarker Discovery, expert researchers in the field detail many of the methods which are now commonly used to study proteomics. These include methods and techniques include both label-free approaches and those that utilize stable isotopes incorporated both during cell growth or added via a chemical reaction once the proteome is extracted from the cell. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Proteomics for Biomarker Discovery seeks to aid scientists in the further study the different sample preparation and data analysis tools used in proteomics today.

Table of contents (24 chapters)

  • Affinity Depletion of Plasma and Serum for Mass Spectrometry-Based Proteome Analysis

    Jaros, Julian A. J. (et al.)

    Pages 1-11

  • Tissue Sample Preparation for Biomarker Discovery

    Suehara, Yoshiyuki (et al.)

    Pages 13-23

  • Subcellular Fractionation for Identification of Biomarkers: Serial Detergent Extraction by Subcellular Accessibility and Solubility

    Hwang, Sun-II (et al.)

    Pages 25-35

  • Analysis of Secreted Proteins

    Severino, Valeria (et al.)

    Pages 37-60

  • Preparation of Human Cerebrospinal Fluid for Proteomics Biomarker Analysis

    Waybright, Timothy J.

    Pages 61-70

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-360-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-359-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5999-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Proteomics for Biomarker Discovery
Editors
  • Ming Zhou
  • Timothy Veenstra
Series Title
Methods in Molecular Biology
Series Volume
1002
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-360-2
DOI
10.1007/978-1-62703-360-2
Hardcover ISBN
978-1-62703-359-6
Softcover ISBN
978-1-4939-5999-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 320
Number of Illustrations and Tables
23 b/w illustrations, 34 illustrations in colour
Topics