Overview
- Includes both well-established and cutting-edge methods and protocols that focus on ionic channel function
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 998)
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Table of contents (32 protocols)
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Front Matter
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Cloning and Heterologous Expression of Ion Channel Genes
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Front Matter
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Electrophysiological Methods to Study Ion Channel Functions
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Front Matter
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Imaging and Fluorescence Methods to Study Ion Channels
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Front Matter
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Keywords
About this book
Every cell in our bodies contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signalling molecules. Maintenance and precise control of gating within these pathways are fundamental principles of life as these underlie basic cellular functions such as communication, contractility, and metabolism. In Ion Channels: Methods and Protocols, Second Edition, experts in the field contribute chapters that focus on the strategies, approaches, methods, and protocols for studying a large family of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Using practical examples from the cutting-edge current research, this volume will take a look back at the major methods and approaches that aided the progress toward the current understanding of ion channel function, structural design, and biological roles. The volume also aims to look forward and identify approaches that will lead us to future discoveries. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Ion Channels: Methods and Protocols, Second Edition will greatly assist researchers searching for specific methodology in studying ion channels on the path toward a greater understanding of these key biological features.
Editors and Affiliations
Bibliographic Information
Book Title: Ion Channels
Book Subtitle: Methods and Protocols
Editors: Nikita Gamper
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-351-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-350-3Published: 26 March 2013
Softcover ISBN: 978-1-4939-6009-5Published: 23 August 2016
eBook ISBN: 978-1-62703-351-0Published: 26 March 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 440
Topics: Cell Biology, Membrane Biology