Methods in Molecular Biology

Ion Channels

Methods and Protocols

Editors: Gamper, Nikita (Ed.)

  • Includes both well-established and cutting-edge methods and protocols that focus on ionic channel function 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-351-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-350-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Every cell in our bodies contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signalling molecules. Maintenance and precise control of gating within these pathways are fundamental principles of life as these underlie basic cellular functions such as communication, contractility, and metabolism.  In Ion Channels: Methods and Protocols, Second Edition, experts in the field contribute chapters that focus on the strategies, approaches, methods, and protocols for studying a large family of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Using practical examples from the cutting-edge current research, this volume will take a look back at the major methods and approaches that aided the progress toward the current understanding of ion channel function, structural design, and biological roles. The volume also aims to look forward and identify approaches that will lead us to future discoveries. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Ion Channels: Methods and Protocols, Second Edition will greatly assist researchers searching for specific methodology in studying ion channels on the path toward a greater understanding of these key biological features.

Table of contents (32 chapters)

  • Approaches to Cloning of Pain-Related Ion Channel Genes

    Akopian, Armen N.

    Pages 3-19

  • Mammalian Expression Systems and Transfection Techniques

    Laurenti, Daunia (et al.)

    Pages 21-32

  • Use of Escherichia coli for the Production and Purification of Membrane Proteins

    Postis, Vincent G. L. (et al.)

    Pages 33-54

  • Transient Overexpression of Genes in Neurons Using Nucleofection

    Kirton, Hannah M. (et al.)

    Pages 55-64

  • Viral Gene Delivery: Optimized Protocol for Production of High Titer Lentiviral Vectors

    Hewinson, James (et al.)

    Pages 65-75

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-351-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-350-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Ion Channels
Book Subtitle
Methods and Protocols
Editors
  • Nikita Gamper
Series Title
Methods in Molecular Biology
Series Volume
998
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-351-0
DOI
10.1007/978-1-62703-351-0
Hardcover ISBN
978-1-62703-350-3
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 440
Topics