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Chemical Neurobiology

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Matthew R. Banghart

    1. , Department of Neurobiology, Harvard Medical School, Boston, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 995)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Chemical Probes of Membrane Protein Structure and Function

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Engineering K+ Channels Using Semisynthesis

      • Alexander G. Komarov, Corey A. Costantino, Francis I. Valiyaveetil
      Pages 3-17

    3. Chemical Derivatization and Purification of Peptide-Toxins for Probing Ion Channel Complexes

      • Zhengmao Hua, William R. Kobertz
      Pages 19-30

    4. Using Yeast to Study Potassium Channel Function and Interactions with Small Molecules

      • Sviatoslav N. Bagriantsev, Daniel L. Minor Jr.
      Pages 31-42

    5. A FLIPR Assay for Evaluating Agonists and Antagonists of GPCR Heterodimers

      • Jessica H. Harvey, Richard M. van Rijn, Jennifer L. Whistler
      Pages 43-54

  3. Photochemical Control of Protein and Cellular Function

    1. Front Matter

      Pages 55-55
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    2. Characterizing Caged Molecules Through Flash Photolysis and Transient Absorption Spectroscopy

      • Joseph P. Y. Kao, Sukumaran Muralidharan
      Pages 57-77

    3. Characterization of One- and Two-Photon Photochemical Uncaging Efficiency

      • Alexandre Specht, Frederic Bolze, Jean Francois Nicoud, Maurice Goeldner
      Pages 79-87

    4. Photochromic Potassium Channel Blockers: Design and Electrophysiological Characterization

      • Alexandre Mourot, Timm Fehrentz, Richard H. Kramer
      Pages 89-105

    5. A 1H NMR Assay for Measuring the Photostationary States of Photoswitchable Ligands

      • Matthew R. Banghart, Dirk Trauner
      Pages 107-120

    6. Developing a Photoreactive Antagonist

      • Pamela M. England
      Pages 121-129

  4. Chemical Probes for Imaging in the Nervous System

    1. Front Matter

      Pages 131-131
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    2. Development and In Vitro Characterization of Ratiometric and Intensity-Based Fluorescent Ion Sensors

      • Laurel A. Cooley, Vladimir V. Martin, Kyle R. Gee
      Pages 133-145

    3. Characterization of Voltage-Sensitive Dyes in Living Cells Using Two-Photon Excitation

      • Corey D. Acker, Leslie M. Loew
      Pages 147-160

    4. Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor

      • Ruud Hovius
      Pages 161-178

    5. Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots

      • Qi Zhang
      Pages 179-191

    6. Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI

      • Philip A. Romero, Mikhail G. Shapiro, Frances H. Arnold, Alan Jasanoff
      Pages 193-205

  5. Back Matter

    Pages 207-219
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Keywords

About this book

Many advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion channel structure to neural network dynamics. Recent advances in photochemistry, microscopy, and protein engineering have triggered a surge in the development and application of these interdisciplinary techniques. Chemical Neurobiology: Methods and Protocols assists with the design, characterization and validation of new chemical tools for neurobiology by providing detailed protocols of procedures and assays deemed essential for the successful development and implementation of such tools. Divided into three sections, topics cover chemical probes of membrane protein structure and function, photochemical control of protein and cellular function, and chemical probes for imaging in the nervous system. Written in the successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Chemical Neurobiology: Methods and Protocols serves scientists at many levels, including students aiming to expand their perspective, laboratory researchers seeking technical guidance, and established investigators looking for creative solutions to their research problems in molecular, cellular and systems neuroscience. 

Editors and Affiliations

  • , Department of Neurobiology, Harvard Medical School, Boston, USA

    Matthew R. Banghart

Bibliographic Information

  • Book Title: Chemical Neurobiology

  • Book Subtitle: Methods and Protocols

  • Editors: Matthew R. Banghart

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-345-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-344-2Published: 16 March 2013

  • Softcover ISBN: 978-1-4939-5782-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-345-9Published: 15 March 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 202

  • Topics: Neurosciences, Neurobiology

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