Methods in Molecular Biology

Glycosylation Engineering of Biopharmaceuticals

Methods and Protocols

Editors: Beck, Alan (Ed.)

  • Presents detailed protocols on the production and characterization of glycoproteins and glyco-engineered biopharmaceuticals
  • Contains a special focus on monoclonal antibodies (mAbs)
  • Features expert tips and key implementation advice to guarantee successful results
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-327-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-326-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins.  In Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols, experts in the field provide readers with production and characterization protocols of glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. The volume is divided in four complementary parts dealing with glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complexes characterization, and PK/PD assays for therapeutic antibodies.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols serves as an ideal guide for scientists striving to push forward the exciting field of engineered biopharmaceuticals.

Reviews

From the reviews:

“The book is intended as a reference manual of protocols and best practices for the practicing bench scientist. The authors do a marvelous job of covering a wide variety of tools and techniques and bringing them together in one volume. … This collection of protocols will find an audience with experimentalists in the biopharmaceutical industry as well as various academic labs. … Overall, I would strongly recommend this book … .” (Bibhash Mukhopadhyay, Frontiers in Pharmacology, September, 2013)

“This book … useful as it details in one place the current state of art technologies and techniques for antibodies. I enjoyed reading the introductions to each chapter, which were accessible and gave an historical setting and overall aim for the methodologies that are set out in the chapter. … this book offers a good reference guide for those of us working in this area.” (Sian Estdale, BTS Newsletter – The British Toxicology Society, Issue 43, Winter, 2013)

Table of contents (22 chapters)

  • Engineering of Therapeutic and Diagnostic O-Glycans on Recombinant Mucin-Type Immunoglobulin Fusion Proteins Expressed in CHO Cells

    Lindberg, Linda (et al.)

    Pages 3-17

  • Engineering a Human-Like Glycosylation to Produce Therapeutic Glycoproteins Based on 6-Linked Sialylation in CHO Cells

    Maï, Nassimal El (et al.)

    Pages 19-29

  • Glycoengineered Pichia-Based Expression of Monoclonal Antibodies

    Zha, Dongxing

    Pages 31-43

  • N-Glycosylation Humanization for Production of Therapeutic Recombinant Glycoproteins in Saccharomyces cerevisiae

    Arico, Christelle (et al.)

    Pages 45-57

  • Engineering the Baculovirus Genome to Produce Galactosylated Antibodies in Lepidopteran Cells

    Juliant, Sylvie (et al.)

    Pages 59-77

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-327-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-326-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Glycosylation Engineering of Biopharmaceuticals
Book Subtitle
Methods and Protocols
Editors
  • Alan Beck
Series Title
Methods in Molecular Biology
Series Volume
988
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-327-5
DOI
10.1007/978-1-62703-327-5
Hardcover ISBN
978-1-62703-326-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 355
Topics