Methods in Molecular Biology

Cytochrome P450 Protocols

Editors: Phillips, Ian R., Shephard, Elizabeth A., Ortiz de Montellano, Paul R. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
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  • ISBN 978-1-62703-321-3
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  • Immediate eBook download after purchase
Hardcover $169.00
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  • ISBN 978-1-62703-320-6
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About this book

Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs (‘de-orphanizing’ CYP substrates). Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics.

Reviews

From the reviews of the third edition:

“With this third edition, the authors and contributors continue to provide what has become a tour de force of bench top manuals for the cytochrome P450 field. … this book should also prove of interest to graduate students and postdoctoral researchers who are new to the field of P450 science, or who would like to expand their research directions. … This stands alone as the premier book for this field, which is sorely lacking in publications of this type.” (Jed N. Lampe, Doody’s Book Reviews, October, 2013)

Table of contents (25 chapters)

  • Bioluminescent Assays for Cytochrome P450 Enzymes

    Auld, Douglas S. (et al.)

    Pages 1-9

  • Simultaneous Determination of Multiple CYP Inhibition Constants using a Cocktail-Probe Approach

    Zientek, Michael (et al.)

    Pages 11-23

  • High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening

    Lim, Kheng B. (et al.)

    Pages 25-50

  • The Synthesis, Characterization, and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites

    McCullough, Christopher (et al.)

    Pages 51-59

  • High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators

    Kenaan, Cesar (et al.)

    Pages 61-69

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-321-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-320-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cytochrome P450 Protocols
Editors
  • Ian R. Phillips
  • Elizabeth A. Shephard
  • Paul R. Ortiz de Montellano
Series Title
Methods in Molecular Biology
Series Volume
987
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-321-3
DOI
10.1007/978-1-62703-321-3
Hardcover ISBN
978-1-62703-320-6
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XII, 319
Topics