Methods in Molecular Biology

Dictyostelium discoideum Protocols

Editors: Eichinger, Ludwig, Rivero, Francisco (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essentail for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
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  • ISBN 978-1-62703-302-2
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Hardcover $179.00
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  • ISBN 978-1-62703-301-5
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Softcover $179.00
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  • ISBN 978-1-4939-5997-6
  • Free shipping for individuals worldwide
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About this book

Dictyostelium discoideum is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. Dictyostelium is also increasingly used for the investigation of human disease genes and the crosstalk between host and pathogen. The availability of the genome sequence at a mouse click together with a whole range of supporting information and resources along with a powerful armoury of molecular genetics techniques have considerably enhanced the experimental attractiveness of D. discoideum in recent years.

 

The second edition of Dictyostelium discoideum Protocols incorporates the most recent developments in a number of fields. The book is divided into four parts. The first part provides an introduction to the amoebozoa and community resources. The second part presents large-scale analysis methods made possible by the completion of the Dictyostelium genome sequence. The third part is dedicated to molecular genetics techniques, cell biological, biochemical and biophysical methods. The chapters in the fourth part describe the use of Dictyostelium as a model system to study vesicle formation, trafficking and infection by bacterial pathogens.

 

Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Dictyostelium discoideum Protocols, Second Edition seeks to aid scientists in the application of innovating techniques to study a range of fundamental biological processes in this attractive model organism.

Table of contents (26 chapters)

  • The Amoebozoa

    Schilde, Christina (et al.)

    Pages 1-15

  • The Model Organism Dictyostelium discoideum

    Bozzaro, Salvatore

    Pages 17-37

  • Comparative Genomics of the Dictyostelids

    Loomis, William F.

    Pages 39-58

  • One Stop Shop for Everything Dictyostelium: dictyBase and the Dicty Stock Center in 2012

    Fey, Petra (et al.)

    Pages 59-92

  • Fluorescent Reporters and Methods to Analyze Fluorescent Signals

    Müller-Taubenberger, Annette (et al.)

    Pages 93-112

Buy this book

eBook $139.00
price for USA (gross)
  • ISBN 978-1-62703-302-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-62703-301-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA
  • ISBN 978-1-4939-5997-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Dictyostelium discoideum Protocols
Editors
  • Ludwig Eichinger
  • Francisco Rivero
Series Title
Methods in Molecular Biology
Series Volume
983
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-302-2
DOI
10.1007/978-1-62703-302-2
Hardcover ISBN
978-1-62703-301-5
Softcover ISBN
978-1-4939-5997-6
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 479
Number of Illustrations and Tables
55 b/w illustrations, 55 illustrations in colour
Topics