Overview
- Features methodologies and approaches that play an important role in the design cycle for strain improvement
- Provides step-by-step detail essential for reproducible results
- Contains key notes and expert implementation advice in order to guarantee success in the lab
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB)
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Table of contents (23 protocols)
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Front Matter
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Modeling and Simulation Tools
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Front Matter
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Systems-Level ‘Omics Tools
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Front Matter
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Systems-Level ‘Omics Tools
Keywords
About this book
With the ultimate goal of systematically and robustly defining the specific perturbations necessary to alter a cellular phenotype, systems metabolic engineering has the potential to lead to a complete cell model capable of simulating cell and metabolic function as well as predicting phenotypic response to changes in media, gene knockouts/overexpressions, or the incorporation of heterologous pathways. In Systems Metabolic Engineering: Methods and Protocols, experts in the field describe the methodologies and approaches in the area of systems metabolic engineering and provide a step-by-step guide for their implementation. Four major tenants of this approach are addressed, including modeling and simulation, multiplexed genome engineering, ‘omics technologies, and large data-set incorporation and synthesis, all elucidated through the use of model host organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Systems Metabolic Engineering: Methods and Protocols serves as an ideal guide for metabolic engineers, molecular biologists, and microbiologists aiming to implement the most recent approaches available in the field.
Editors and Affiliations
Bibliographic Information
Book Title: Systems Metabolic Engineering
Book Subtitle: Methods and Protocols
Editors: Hal S. Alper
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-299-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-298-8Published: 16 February 2013
Softcover ISBN: 978-1-4939-6292-1Published: 30 April 2017
eBook ISBN: 978-1-62703-299-5Published: 15 February 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 474
Number of Illustrations: 14 b/w illustrations, 47 illustrations in colour
Topics: Biotechnology, Microengineering, Metabolomics