Methods in Molecular Biology

Systems Metabolic Engineering

Methods and Protocols

Editors: Alper, Hal S. (Ed.)

  • Features methodologies and approaches that play an important role in the design cycle for strain improvement
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and expert implementation advice in order to guarantee success in the lab
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-299-5
  • Digitally watermarked, DRM-free
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-298-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-4939-6292-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

With the ultimate goal of systematically and robustly defining the specific perturbations necessary to alter a cellular phenotype, systems metabolic engineering has the potential to lead to a complete cell model capable of simulating cell and metabolic function as well as predicting phenotypic response to changes in media, gene knockouts/overexpressions, or the incorporation of heterologous pathways.  In Systems Metabolic Engineering: Methods and Protocols, experts in the field describe the methodologies and approaches in the area of systems metabolic engineering and provide a step-by-step guide for their implementation. Four major tenants of this approach are addressed, including modeling and simulation, multiplexed genome engineering, ‘omics technologies, and large data-set incorporation and synthesis, all elucidated through the use of model host organisms.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Systems Metabolic Engineering: Methods and Protocols serves as an ideal guide for metabolic engineers, molecular biologists, and microbiologists aiming to implement the most recent approaches available in the field.

Table of contents (23 chapters)

  • Genome-Scale Model Management and Comparison

    Pabinger, Stephan (et al.)

    Pages 3-16

  • Automated Genome Annotation and Metabolic Model Reconstruction in the SEED and Model SEED

    Devoid, Scott (et al.)

    Pages 17-45

  • Metabolic Model Refinement Using Phenotypic Microarray Data

    Gawand, Pratish (et al.)

    Pages 47-59

  • Linking Genome-Scale Metabolic Modeling and Genome Annotation

    Blais, Edik M. (et al.)

    Pages 61-83

  • Resolving Cell Composition Through Simple Measurements, Genome-Scale Modeling, and a Genetic Algorithm

    Senger, Ryan S. (et al.)

    Pages 85-101

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-299-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-298-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-4939-6292-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Systems Metabolic Engineering
Book Subtitle
Methods and Protocols
Editors
  • Hal S. Alper
Series Title
Methods in Molecular Biology
Series Volume
985
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-299-5
DOI
10.1007/978-1-62703-299-5
Hardcover ISBN
978-1-62703-298-8
Softcover ISBN
978-1-4939-6292-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 474
Number of Illustrations and Tables
14 b/w illustrations, 47 illustrations in colour
Topics