Methods in Molecular Biology

Enzyme Engineering

Methods and Protocols

Editors: Samuelson, James C. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-62703-293-3
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About this book

Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs.

Table of contents (18 chapters)

  • A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability In Vivo

    Foit, Linda (et al.)

    Pages 1-20

  • Determining Enzyme Kinetics via Isothermal Titration Calorimetry

    Demarse, Neil A. (et al.)

    Pages 21-30

  • GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria

    Paley, Olga (et al.)

    Pages 31-44

  • Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA-Binding and Cleavage Properties

    Baxter, Sarah K. (et al.)

    Pages 45-61

  • TAL Effector Nuclease (TALEN) Engineering

    Li, Ting (et al.)

    Pages 63-72

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-293-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-292-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Enzyme Engineering
Book Subtitle
Methods and Protocols
Editors
  • James C. Samuelson
Series Title
Methods in Molecular Biology
Series Volume
978
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
Distribution Rights
Distribution rights for India: Mehul Book Sales, Mumbai, India
eBook ISBN
978-1-62703-293-3
DOI
10.1007/978-1-62703-293-3
Hardcover ISBN
978-1-62703-292-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 252
Topics