Methods in Molecular Biology

Immune Homeostasis

Methods and Protocols

Editors: Snow, Andrew L., Lenardo, Michael J. (Eds.)

  • Focuses on experimental techniques for measuring and analyzing immune cell dynamics
  • Emphasizes lymphocyte programmed cell deaths in different contexts
  • Features expert tips and key implementation advice to guarantee successful results
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eBook $109.00
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  • ISBN 978-1-62703-290-2
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Hardcover $139.00
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valid through November 5, 2017
  • ISBN 978-1-62703-289-6
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  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
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  • ISBN 978-1-4939-5968-6
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About this book

Many questions remain unresolved as researchers continue to characterize and define the nature of normal immune homeostasis and determine how these processes are dysregulated in immunodeficiency, as well as in autoimmune and lymphoproliferative disorders.  Immune Homeostasis: Methods and Protocols focuses on experimental techniques for measuring and analyzing immune cell dynamics, with a particular emphasis on examining lymphocyte programmed cell death in different contexts.  With contributions by leading experts in the field, the collection examines detailed protocols for studying various pathways of apoptosis and necrosis in different types of hematopoietic cells, both in vitro and in vivo, methods for studying the maintenance of lymphocyte populations in the steady-state or following infectious challenges in both mice and humans, and technical insights into state-of-the-art genomics tools, among other topics.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Valuable and easy to use, Immune Homeostasis: Methods and Protocols serves as an important toolkit for basic and clinical scientists interested in examining various aspects of immune homeostasis in both normal and disease-related contexts in order to further study the dynamic processes that contribute to homeostasis of the immune system.

Table of contents (16 chapters)

  • Quantitating Lymphocyte Programmed Cell Death In Vitro Using Simple Kill Assays

    Zheng, Lixin

    Pages 1-14

  • Fluorescence-Activated Cell Sorting-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death in Activated, Primary Human T Cells

    Katz, Gil (et al.)

    Pages 15-23

  • Evaluation of IL-2-Withdrawal-Induced Apoptosis in Human T Lymphocytes

    Oliveira, Joao Bosco

    Pages 25-31

  • Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multiparameter Fluorescence-Activated Cell Sorting-Based Technique

    Lo, Bernice (et al.)

    Pages 33-41

  • Visualization of Fas-Mediated Death-Inducing Signaling Complex Formation by Immunoprecipitation

    Cordeiro, Nelia (et al.)

    Pages 43-49

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-290-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-62703-289-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5968-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Immune Homeostasis
Book Subtitle
Methods and Protocols
Editors
  • Andrew L. Snow
  • Michael J. Lenardo
Series Title
Methods in Molecular Biology
Series Volume
979
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-290-2
DOI
10.1007/978-1-62703-290-2
Hardcover ISBN
978-1-62703-289-6
Softcover ISBN
978-1-4939-5968-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 215
Topics