Methods in Molecular Biology

Virus-Induced Gene Silencing

Methods and Protocols

Editors: Becker, Annette (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-62703-278-0
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  • Immediate eBook download after purchase
Hardcover $139.00
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  • ISBN 978-1-62703-277-3
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About this book

Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions.

Table of contents (16 chapters)

  • Virus-Induced Gene Silencing (VIGS) in Plants: An Overview of Target Species and the Virus-Derived Vector Systems

    Lange, Matthias (et al.)

    Pages 1-14

  • Rationale for Developing New Virus Vectors to Analyze Gene Function in Grasses Through Virus-Induced Gene Silencing

    Ramanna, Hema (et al.)

    Pages 15-32

  • Virus-Induced Gene Silencing for Rice Using Agroinoculation

    Purkayastha, Arunima (et al.)

    Pages 33-45

  • Utilizing Virus-Induced Gene Silencing for the Functional Characterization of Maize Genes During Infection with the Fungal Pathogen Ustilago maydis

    Linde, Karina (et al.)

    Pages 47-60

  • Analysis of Developmental Control Genes Using Virus-Induced Gene Silencing

    Geuten, Koen (et al.)

    Pages 61-69

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-278-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-62703-277-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Virus-Induced Gene Silencing
Book Subtitle
Methods and Protocols
Editors
  • Annette Becker
Series Title
Methods in Molecular Biology
Series Volume
975
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-278-0
DOI
10.1007/978-1-62703-278-0
Hardcover ISBN
978-1-62703-277-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 221
Topics