Methods in Molecular Biology

Lipid-Protein Interactions

Methods and Protocols

Editors: Kleinschmidt, Jörg H. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane proteins with lipids. Lipid-Protein Interactions: Methods and Protocols provides a selection of protocols to examine protein-lipid interactions, membrane and membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer and lipid properties. The methods described here are all actively used, complementary, and necessary to obtain comprehensive information about membrane structure and function. They include label-free approaches, imaging techniques and spectroscopic methodologies. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Lipid-Protein Interactions: Methods and Protocols seeks to serve both professional and novices with its wide range of the methods frequently used in this area of research.

Table of contents (19 chapters)

  • Quartz Crystal Microbalances as Tools for Probing Protein–Membrane Interactions

    Nielsen, Søren B. (et al.)

    Pages 1-21

  • Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipid Membranes

    Hodnik, Vesna (et al.)

    Pages 23-36

  • Probing the Thermodynamics of Protein–Lipid Interactions by Isothermal Titration Calorimetry

    Swamy, Musti J. (et al.)

    Pages 37-53

  • Differential Scanning Calorimetry of Protein–Lipid Interactions

    Cañadas, Olga (et al.)

    Pages 55-71

  • Atomic Force Microscopy and Spectroscopy to Probe Single Membrane Proteins in Lipid Bilayers

    Sapra, K. Tanuj

    Pages 73-110

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-275-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-274-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Lipid-Protein Interactions
Book Subtitle
Methods and Protocols
Editors
  • Jörg H. Kleinschmidt
Series Title
Methods in Molecular Biology
Series Volume
974
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-275-9
DOI
10.1007/978-1-62703-275-9
Hardcover ISBN
978-1-62703-274-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 464
Topics