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Synthetic Messenger RNA and Cell Metabolism Modulation

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Peter M. Rabinovich

    1. The Anlyan Center, Department of Genetics, Yale School of Medicine, New Haven, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 969)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. mRNA Synthesis, Purification, and Transfer

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Cell Engineering with Synthetic Messenger RNA

      • Peter M. Rabinovich, Sherman M. Weissman
      Pages 3-28

    3. In Vitro Transcription of Long RNA Containing Modified Nucleosides

      • Norbert Pardi, Hiromi Muramatsu, Drew Weissman, Katalin Karikó
      Pages 29-42

    4. HPLC Purification of In Vitro Transcribed Long RNA

      • Drew Weissman, Norbert Pardi, Hiro Muramatsu, Katalin Karikó
      Pages 43-54

    5. Synthetic mRNAs with Superior Translation and Stability Properties

      • Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz et al.
      Pages 55-72

    6. Nonviral, Cationic Lipid-Mediated Delivery of mRNA

      • James G. Hecker
      Pages 73-88

    7. Light-Induced mRNA Transfection

      • Sigurd Leinæs Bøe, Eivind Hovig
      Pages 89-100

    8. Engineering B Cells with mRNA

      • Jaewoo Lee, David Boczkowski, Smita Nair
      Pages 101-110

    9. Programming Human Dendritic Cells with mRNA

      • Jaewoo Lee, David Boczkowski, Smita Nair
      Pages 111-125

    10. Large Volume Flow Electroporation of mRNA: Clinical Scale Process

      • Linhong Li, Cornell Allen, Rama Shivakumar, Madhusudan V. Peshwa
      Pages 127-138

    11. Retrovirus-Based mRNA Transfer for Transient Cell Manipulation

      • Melanie Galla, Axel Schambach, Christopher Baum
      Pages 139-161

    12. mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells

      • Frank Breinig, Tanja Breinig, Manfred J. Schmitt
      Pages 163-184

  3. Modulation and Monitoring of Cell Metabolism

    1. Front Matter

      Pages 185-185
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  4. Modulation and monitoring of cell metabolism

    1. Nonviral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy

      • Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, Hinrich Abken
      Pages 187-201

    2. Natural Killer Cell Reprogramming with Chimeric Immune Receptors

      • Noriko Shimasaki, Dario Campana
      Pages 203-220

    3. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA

      • Peizhe Wang, Jie Na
      Pages 221-233

    4. Antitumor Vaccination with Synthetic mRNA: Strategies for In Vitro and In Vivo Preclinical Studies

      • Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, Ugur Sahin
      Pages 235-246

    5. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA

      • Chantal Pichon, Patrick Midoux
      Pages 247-274

    6. Analysis of Survivin-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA

      • Özcan Met, Inge Marie Svane
      Pages 275-292
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Keywords

About this book

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that  can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing  the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery.  Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.

Editors and Affiliations

  • The Anlyan Center, Department of Genetics, Yale School of Medicine, New Haven, USA

    Peter M. Rabinovich

Bibliographic Information

  • Book Title: Synthetic Messenger RNA and Cell Metabolism Modulation

  • Book Subtitle: Methods and Protocols

  • Editors: Peter M. Rabinovich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-260-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-259-9Published: 08 January 2013

  • Softcover ISBN: 978-1-4939-5967-9Published: 23 August 2016

  • eBook ISBN: 978-1-62703-260-5Published: 09 January 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 323

  • Topics: Human Genetics

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