Methods in Molecular Biology

Synthetic Messenger RNA and Cell Metabolism Modulation

Methods and Protocols

Editors: Rabinovich, Peter M. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-260-5
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Hardcover $169.00
price for USA
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  • ISBN 978-1-62703-259-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
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valid through November 5, 2017
  • ISBN 978-1-4939-5967-9
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About this book

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that  can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing  the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery.  Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.

Table of contents (19 chapters)

  • Cell Engineering with Synthetic Messenger RNA

    Rabinovich, Peter M. (et al.)

    Pages 3-28

  • In Vitro Transcription of Long RNA Containing Modified Nucleosides

    Pardi, Norbert (et al.)

    Pages 29-42

  • HPLC Purification of In Vitro Transcribed Long RNA

    Weissman, Drew (et al.)

    Pages 43-54

  • Synthetic mRNAs with Superior Translation and Stability Properties

    Grudzien-Nogalska, Ewa (et al.)

    Pages 55-72

  • Nonviral, Cationic Lipid-Mediated Delivery of mRNA

    Hecker, James G.

    Pages 73-88

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-260-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-62703-259-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-5967-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Synthetic Messenger RNA and Cell Metabolism Modulation
Book Subtitle
Methods and Protocols
Editors
  • Peter M. Rabinovich
Series Title
Methods in Molecular Biology
Series Volume
969
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-260-5
DOI
10.1007/978-1-62703-260-5
Hardcover ISBN
978-1-62703-259-9
Softcover ISBN
978-1-4939-5967-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 323
Topics